Study of Biological Effect of Carbon Nanotube by Intratracheal Instillation to Rats.

碳纳米管气管灌注对大鼠的生物学效应研究。

• 批准号: 16590494
• 负责人: OGAMI Akira
• 金额: $ 2.18万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590494/
• 关键词: nanoparticle; carbon nanotube; intratracheal instillation; BAL; carbon graphite; pulmonary fibrosis; lung cancer; 気管支肺胞洗浄液; カーボングアファイト

This study aimed to evaluate the pulmonary effect of carbon nanotube (CNT) by intratracheal instillation to rats. Nanoparticles have specific optical and electric properties. In general, nanoparticles have a tendency to agglomerate, therefore, it is necessary to prevent the agglomeration of the nanoparticles as possible when we evaluate the toxicity. Before instillation, we confirmed the dispersion of the nanotubes with the surface active agent (Triton X) under scanning electron microscopy.We instilled CNT (with 0.5% Triton X saline) intratracheally at the concentration of 40 μg to Wistar male rats (8 weeks old). Crystalline silica (median diameter 0.8 μm) was also instilled as a reference material. Control rats were instilled only saline with Triton X. Autopsy was done at 3days, 1 week, 1 month, 3 months, and 6 months after instillation. Bronchoalveolar lavage fluid (BALF), blood, lung tissue were extracted from each rat.Total cell counts in BALF showed that the number of cells was increased at the early phase after instillation of CNT, however, no significant difference were observed at 3months or later time period. For the percentage of PMN, the significant increase was observed in CNT and silica instilled groups at 3 days and at 3 months later. We examined pulmonary inflammation in each by the point counting method (PCM). As six images digitally captured under the light microscopy were examined per specimen, 1800 points (300 points x 6 images) were examined in each lung section. We counted the number of points that had accumulated inflammatory cells and the number of air spaces. There were no significant difference in inflammatory changes in lung section determined by point counting method between exposure group (CNT and crystalline silica) and control. In conclusion, CNT did not show the severe pulmonary effect by intratracheal instillation to rats at the concentration of 40 μg.

本研究旨在通过气管内滴注评估碳纳米管（CNT）对大鼠的肺效应。纳米粒子具有特定的光学和电学特性。一般来说，纳米粒子有团聚的倾向，因此，在评价毒性时需要尽可能防止纳米粒子的团聚。滴注前，我们在扫描电子显微镜下确认纳米管与表面活性剂（Triton X）的分散。我们将CNT（含0.5％Triton X盐水）以40μg的浓度注入Wistar雄性大鼠（8周龄）气管内。还滴注了结晶二氧化硅（中位直径 0.8 μm）作为参考物质。对照大鼠仅用Triton X滴注盐水。在滴注后3天、1周、1个月、3个月和6个月进行尸检。抽取每只大鼠的支气管肺泡灌洗液(BALF)、血液、肺组织。BALF中的总细胞计数显示，在滴注CNT后的早期，细胞数量有所增加，但在3个月及以后，没有观察到显着差异。对于PMN的百分比，在CNT和二氧化硅滴注组中在3天和3个月后观察到显着增加。我们通过点计数法（PCM）检查了每个患者的肺部炎症。由于每个样本要检查光学显微镜下数字捕获的 6 个图像，因此每个肺切片检查了 1800 个点（300 点 x 6 个图像）。我们计算了积累炎症细胞的点的数量和气隙的数量。点计数法测定暴露组（CNT和结晶二氧化硅）与对照组的肺切片炎症变化无显着差异。总之，40μg浓度的CNT对大鼠气管内滴注并未表现出严重的肺部效应。

项目成果

職業性肺疾患のワイポイントアドバイス

关于职业性肺病的重要建议

• 发表时间: 2006
• 期刊: 日本医事新報 4285.5/12
• 作者: Oyama T;et al.;森本 泰夫
• 通讯作者: 森本 泰夫

酸化ニッケル、結晶質シリカ、二酸化チタンの経気道生体影響

氧化镍、结晶二氧化硅和二氧化钛的呼吸生物效应

• 发表时间: 2005
• 期刊: エアロゾル研究 20・3
• 作者: Fukuda Y;Nakao H;Imai H;大神 明
• 通讯作者: 大神 明

Pulmonary Effect of Nickel Oxide, Crystalline silica and Titanium Dioxide.

氧化镍、结晶二氧化硅和二氧化钛的肺效应。

• 发表时间: 2005
• 期刊: Earozoru Kenkyu 20-3
• 作者: Ogami;A.;et al.
• 通讯作者: et al.

The effect of lung burden on biopersistence and pulmonary effects in rats exposed to potassium octatitanate whiskers by intratracheal instillation

• DOI: 10.1539/joh.48.44
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF OCCUPATIONAL HEALTH
• 影响因子: 3
• 作者: Oyabu, T;Yamoto, H;Tanaka, I
• 通讯作者: Tanaka, I

Phospholipid concentration in lung lavage fluid as biomarker for pulmonary fibrosis

• DOI: 10.1080/08958370500516200
• 发表时间: 2006-05-01
• 期刊: INHALATION TOXICOLOGY
• 影响因子: 2.1
• 作者: Kuroda, K;Morimoto, Y;Tanaka, I
• 通讯作者: Tanaka, I