Development and establishment and of mitochondrial DNA screening system based on haplogroup

基于单倍群的线粒体DNA筛选系统的开发与建立

• 批准号: 16590532
• 负责人: NATA Masayuki
• 金额: $ 1.54万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590532/
• 关键词: mitochondria; SNP; haplogroup; SNaPshot; TaqMan PCR; suspension array technology; Taqan PCR; 9bp欠失; ハプログループ分類; 一塩基プライマー伸長反応; Multiplex

In this study, mitochondrial DNA haplogrouping by SNaPshot-single nucleotide extension reaction, TaqMan PCR method, and suspension array technology had been carried out.In SNaPshot-single nucleotide extension reaction, to detect the 3172^<nd>, 3206^<th>, 3338^<th>, 3394^<th>, 4715^<th>, 4761^<st>, 4793^<rd>, 4833^<rd>, 5147^<th>, 9824^<th>, 9923^<rd>, 10310^<th>, 10321^<st>, 10345^<th>, 10373^<rd>, 10397^<th> and 10410^<th> single nucleotide polymorphisms of mitochondrial DNA, primers were designed, and then, multiplex detection system was developed.In TaqMan PCR method, TaqMan probes and primers for the 10398^<th> and 10400^<th> nucleotide to distinguish between haplogroup M and N, 4833^<rd> to detect haplogroup G, 5178^<th> to detect D, 15487^<th> to detect M8a・C4・CZ, 6455^<th> to detect M7 were designed. Rapidly detection of these 6 SNPs was developed.Luminex system-suspension array technology was carried out for mitochondrial DNA screening. In this study, IB, IC1, IC2 and ID in HV I, IIA1, IIA2, IIB and IIC were analyzed. In 105 unrelated Japanese, 19 SNPs were detected, and 50 kinds of mitochondrial DNA were detected.

本研究通过SNaPshot-单核苷酸延伸反应、TaqMan PCR方法和悬浮阵列技术对线粒体DNA进行单倍群分析。在SNaPshot-单核苷酸延伸反应中，检测到3172^<nd>、3206^<th>、3338^<th>、3394^<th>、4715^<th>、 4761^<st>、4793^<rd>、4833^<rd>、5147^<th>、9824^<th>、9923^<rd>、10310^<th>、10321^<st>、10345^<th>、10373^<rd>、10397^<th> 和10410^<第>个单核苷酸 TaqMan PCR方法中，TaqMan探针和引物针对第10398^<th>和10400^<th>核苷酸区分单倍群M和N，4833^<rd>检测单倍群G，5178^<th>检测D， 设计了检测M8a・C4・CZ的15487^<th>、检测M7的6455^<th>。开发了这6个SNP的快速检测方法。采用Luminex系统-悬浮阵列技术进行线粒体DNA筛查。本研究对HV I、IIA1、IIA2、IIB和IIC中的IB、IC1、IC2和ID进行了分析。在105名无血缘关系的日本人中，检测到19个SNP，检测到50种线粒体DNA。

项目成果

法医学と遺伝学的検査

法医和基因检测

• 发表时间: 2005
• 期刊: 日本臨床 63
• 作者: 吉村典子;中塚喜義;中村利孝;折茂肇;那谷 雅之
• 通讯作者: 那谷 雅之

The relationship of a high level of serum beta-hydroxybutyrate to cause of death

• DOI: 10.1016/j.legalmed.2005.01.003
• 发表时间: 2005-05-05
• 期刊: Legal Medicine
• 影响因子: 1.5
• 作者: Kanetake, Jun;Kanawaku, Yoshimasa;Funayama, Masato
• 通讯作者: Funayama, Masato

12 Y-chromosomal STR haplotypes in Japanese.

日语中的 12 个 Y 染色体 STR 单倍型。

• 发表时间: 2006
• 期刊: Forensic Sci Int. 158
• 作者: Hashiyada M;Nagashima T;Itakura Y;Sakai J;Kanawaku Y;Nata M;Funayama M.
• 通讯作者: Funayama M.

Simulation of kinship testing using Monte Carlo method.

使用蒙特卡罗方法模拟亲属关系测试。

• 发表时间: 2005
• 期刊: DNA polymorphism. 13
• 作者: Sakai J;Nata M;Hashiyada M;Funayama M.
• 通讯作者: Funayama M.

Utility of DNA analyses in personal identification of a dismembered corpse.

DNA 分析在肢解尸体个人身份识别中的应用。

• 发表时间: 2004
• 期刊: Acta Crim Japan Vol.70
• 作者: Nata M;Hashiyada M;Mimasaka S;Yajima Y;Funayama M
• 通讯作者: Funayama M