Development and establishment and of mitochondrial DNA screening system based on haplogroup

在本研究中，SNaPshot-单核苷扩增反应的线粒体DNA芯片组，TaqMan PCR方法，和悬浮阵列技术已经被携带出来，在SNaPshot-单核苷延伸反应中，以检测3172 ^，3206 ^，3338 ^，3394 ^，4715 ^，4761 ^，4793 ^，4833 ^，5147 ^，9824 ^，9923 ^，10310 ^，10321 ^，10345 ^，10373 ^，10397 ^和10410 ^单核苷酸多态性的线粒体DNA，引物是设计的，然后，多路检测系统已经开发出来，在TaqMan PCR方法中，TaqMan探针和引物用于10398 ^和10400 ^核肽在抗原组M和N之间分离。4833 ^to detect haplog\G，5178 ^to detect D，15487 ^to detect M8a·C4·CZ，6455 ^to detect M7 were designed。<nd><th><th><th><th><st><rd><rd><th><th><rd><th><st><th><rd><th><th><th><th><rd><th><th><th>迅速地检测到了这6个SNPs，Luminex系统悬挂阵列技术已被用于线粒体DNA筛选。在本研究中，IB，IC1，IC2和ID在HV I，IIA1，IIA2，IIB和IIC中进行了分析。在105个不相关的日本人中，19个SNPs被检测到，50个线粒体DNA被检测到。

In this study, mitochondrial DNA haplogrouping by SNaPshot-single nucleotide extension reaction, TaqMan PCR method, and suspension array technology had been carried out.In SNaPshot-single nucleotide extension reaction, to detect the 3172^<nd>, 3206^<th>, 3338^<th>, 3394^<th>, 4715^<th>, 4761^<st>, 4793^<rd>, 4833^<rd>, 5147^<th>, 9824^<th>, 9923^<rd>, 10310^<th>, 10321^<st>, 10345^<th>, 10373^<rd>, 10397^<th> and 10410^<th> single nucleotide polymorphisms of mitochondrial DNA, primers were designed, and then, multiplex detection system was developed.In TaqMan PCR method, TaqMan probes and primers for the 10398^<th> and 10400^<th> nucleotide to distinguish between haplogroup M and N, 4833^<rd> to detect haplogroup G, 5178^<th> to detect D, 15487^<th> to detect M8a・C4・CZ, 6455^<th> to detect M7 were designed. Rapidly detection of these 6 SNPs was developed.Luminex system-suspension array technology was carried out for mitochondrial DNA screening. In this study, IB, IC1, IC2 and ID in HV I, IIA1, IIA2, IIB and IIC were analyzed. In 105 unrelated Japanese, 19 SNPs were detected, and 50 kinds of mitochondrial DNA were detected.