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Study on the biotinidase competitive inhibition constant in liver cancer, and its control by a sugar chain

肝癌生物素酶竞争性抑制常数及其糖链调控的研究

基本信息

批准号：
16590575
负责人：
NAGAMINE Takeaki
金额：
$ 2.3万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590575/
关键词：
fucoidan cultured hepatoma cell line biotinidase α fetoprotein 細胞周期 microPIXE 培養肝癌細胞 αフェトピロテイン

项目摘要

1. Anti-tumor effect of fucoidan on cultured hepatoma cell linesAccording to the results of MTT assay, natural fucoidan showed an inhibitory effect in dose dependent manner on Hep G2 cells, Huh7 cells, and Chang cells (normal hepatocyte). The inhibitory effect of fucoidan on cell proliferation is similar among these three cell lines. Large molecular fucoidan exerted a stronger inhibitory effect on Chang cells and Huh7cells than in HepG2 cells. Judging from TUNEL stain using HepG2 cells and Chang cells, apoptosis induction by natural fucoidan was milder than that by nigero-oligosaccharide.2. Effect of fucoidan on a fetoprotein production (a tumor marker for hepatocellular carcinoma)HepG2cells and Huh7cells were treated with various concentrations of fucoidan (0%, 1%, 2%, 4%, 10%, 20%), and medium a fetoprotein levels were determined after 24 h after treatment. Consequently, fucoidan. reduced medium α fetoprotein levels of HepG2 cells in a dose dependent manner. Comparing to native and small molecular fucoidans, large molecular fucoidan reduced predominantly α fetoprotein levels. Medium α fetoprotein levels were decrease by natural, large molecular and small molecular fucoidans in dose dependent manner. The western blotting of α fetoprotein in Huh7 cells showed marked reduction of α fetoprotein by natural fucoidan, but not by nigero-oligosaccharide. On the other hand, nigero-oligosaccharide did not decrease α fetoprotein levels of Huh? cells and HepG2 cells.3. Changes of biotinidase enzyme activity by fucoidanNatural fucoidan markedly decreased biotinidase Ki of HepG2 cells and Huh7 cells.These data suggested that fucoidan may demonstrate anti-tumor activity through modulating the enzyme kinetics of biotinidase.

1.岩藻糖胶对体外培养的人肝癌细胞株的抗肿瘤作用实验结果表明，天然岩藻糖胶对Hep G2细胞、Huh7细胞和Chang细胞(正常肝细胞)均有抑制作用，且呈剂量依赖性。岩藻糖胶对细胞增殖的抑制作用在三种细胞系中相似。大分子岩藻糖胶对Chang细胞和HuH7细胞的抑制作用强于对HepG2细胞的抑制作用。从HepG2细胞和Chang细胞的TUNEL染色来看，天然岩藻糖胶诱导的细胞凋亡比黑寡糖诱导的要温和。用不同浓度的岩藻糖胶(0%、1%、2%、4%、10%、20%)处理人肝癌细胞株HepG2和HuH7细胞，24 h后测定细胞培养液中甲胎蛋白水平。因此，岩藻多糖胶。以剂量依赖的方式降低细胞培养上清液中α的胎蛋白水平。与天然和小分子岩藻糖胶相比，大分子岩藻糖胶主要降低α胎儿蛋白水平。天然岩藻糖胶、大分子岩藻糖胶和小分子岩藻糖胶均能剂量依赖性地降低中α胎儿蛋白水平。免疫印迹结果显示，天然岩藻糖胶可显著降低HUH7细胞的α胎蛋白表达，而黑寡糖对α表达无明显影响。另一方面，黑寡糖不能降低HUH？α的胎蛋白水平。细胞和HepG2细胞。岩藻糖胶天然岩藻糖胶对生物素酶活力的影响明显降低了HepG2细胞和HuH7细胞的生物素酶活力，提示岩藻糖胶可能通过调节生物素酶的酶动力学表现出抗肿瘤活性。

项目成果

期刊论文数量（33）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interferon-alpha-induced changes in metallothionein expression in liver biopsies from patients with chronic hepatitis C

干扰素α诱导慢性丙型肝炎患者肝活检中金属硫蛋白表达的变化

DOI：
发表时间：
2005
期刊：
Can J Gastroenterology 19
影响因子：
0
作者：
T Sekiguchi;T Sekiguchi;K Kawai;T Nagamine
通讯作者：
T Nagamine

Affinity chromatographic determination of D-Aspartic acid in the liver cell lines

亲和色谱法测定肝细胞系中D-天冬氨酸

DOI：
发表时间：
期刊：
Trends in Chromatography (in press)
影响因子：
0
作者：
Shao R-X;Otsuka M;Kato N;Taniguchi H;Hoshida Y;Moriyama M;Kawabe T;Omata M.;Taro Kano;K Hayakawa
通讯作者：
K Hayakawa

Autoimmune thrombocytopenia in response to splenectomy in cirrhotic patients with accompanying hepatitis C.

伴有丙型肝炎的肝硬化患者脾切除后出现自身免疫性血小板减少症。

DOI：
发表时间：
2006
期刊：
World J Gastroenterol 12
影响因子：
0
作者：
T Sekiguchi
通讯作者：
T Sekiguchi

Immune thrombocytopenic purpura in patients with hepatitis Cvirus infection

丙型肝炎病毒感染者免疫性血小板减少性紫癜

DOI：
发表时间：
2005
期刊：
Hepato-Gastroenterology 52
影响因子：
0
作者：
吉山直樹;吉山直樹;吉山直樹;吉山直樹;吉山直樹;吉山直樹;T Sekiguchi;T Sekiguchi;K Kawai;T Nagamine;H Nakajima
通讯作者：
H Nakajima

Interferon-alpha-induced changes metallothionein expression in liver biopsies from patients with chronic hepatitis C

干扰素α诱导的慢性丙型肝炎患者肝活检中金属硫蛋白表达的变化

DOI：
发表时间：
2005
期刊：
Can J Gastroenterology 19
影响因子：
0
作者：
Wang Y;Kato N;Amarsanaa J;Dharel N;Otsuka M;Taniguchi H;Kawabe T;Omata M.;T Nagamine
通讯作者：
T Nagamine