Analysis of elemental distribution in erythrocytes using in-air-micro PIXE and its clinical application

贫血常与多种疾病有关，是化疗的副作用。抗癌药物、干扰素治疗和放射治疗引起的贫血变得越来越严重，因此需要开发新的诊断技术工具。本研究建立了红细胞中元素分布的空气中微量PIXE分析方法，首先确定了红细胞样品进行空气中微量PIXE分析的最佳条件，确定了Ortega改良法适用于红细胞样品的调制。从正常志愿者收集的红细胞中，元素图与红细胞形状相吻合，并倾向于形成小而致密的结节。血液透析患者的红细胞碎片比对照组更明显。血液透析患者体内的铁点倾向于部分聚集，并分成一个或两个室。慢性丙型肝炎患者接受干扰素治疗后，各成分分布较厚，分布于大多数红细胞，并呈明显的剂量依赖性。我们的研究表明，在空气中微量PIXE分析红细胞中的元素是有用的，以探讨与血液透析和干扰素治疗相关的贫血的发病机制。

Anemia is often associated with various diseases and occurs as adverse effect of chemotherapy. Anemia complicated by anti-cancer drug, interferon therapy and radiation therapy becomes seriously, therefore development of new tool for diagnostic technique is desired. In this study, we established new analytical method of elemental distribution in erythrocytes using in-air mico-PIXE.At first, we determined the optimum condition of erythrocyte samples for in-air micro-PIXE analysis, confirming that the modified method of Ortega was suitable for modulation of erythrocyte samples. In erythrocytes collected form normal volunteers, elemental maps fit with the erythrocyte shape and tended to form small dense nodules. Erythrocyte fragments were more conspicuous in hemodialysis patients than controls. Hemodialysis patients showed that iron dots tended to aggregate partially and be divided into one or two compartments. In chronic hepatitis C patients received interferon therapy, each element distributed thickly and spread over most erythrocytes, this finding became apparently in dose dependent manner. Our study suggest that analysis of elements in erythrocytes by in-air micro-PIXE is useful to investigate pathogenesis of anemia associated with hemodialysis and interferon therapy.