Analysis of elemental distribution in erythrocytes using in-air-micro PIXE and its clinical application

空气微型PIXE分析红细胞元素分布及其临床应用

• 批准号: 21591545
• 负责人: NAGAMINE Takeaki
• 金额: $ 3万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591545/
• 关键词: 大気マイクロPIXE,貧血; 赤血球; 透析; マイクロPIXE; C型慢性肝炎; 瀉血療法; 鉄; カリウム; 腎性貧血; 大気マイクロPIXE; Mg; 肝硬変

Anemia is often associated with various diseases and occurs as adverse effect of chemotherapy. Anemia complicated by anti-cancer drug, interferon therapy and radiation therapy becomes seriously, therefore development of new tool for diagnostic technique is desired. In this study, we established new analytical method of elemental distribution in erythrocytes using in-air mico-PIXE.At first, we determined the optimum condition of erythrocyte samples for in-air micro-PIXE analysis, confirming that the modified method of Ortega was suitable for modulation of erythrocyte samples. In erythrocytes collected form normal volunteers, elemental maps fit with the erythrocyte shape and tended to form small dense nodules. Erythrocyte fragments were more conspicuous in hemodialysis patients than controls. Hemodialysis patients showed that iron dots tended to aggregate partially and be divided into one or two compartments. In chronic hepatitis C patients received interferon therapy, each element distributed thickly and spread over most erythrocytes, this finding became apparently in dose dependent manner. Our study suggest that analysis of elements in erythrocytes by in-air micro-PIXE is useful to investigate pathogenesis of anemia associated with hemodialysis and interferon therapy.

贫血常与多种疾病有关，是化疗的副作用。抗癌药物、干扰素治疗和放射治疗引起的贫血变得越来越严重，因此需要开发新的诊断技术工具。本研究建立了红细胞中元素分布的空气中微量PIXE分析方法，首先确定了红细胞样品进行空气中微量PIXE分析的最佳条件，确定了Ortega改良法适用于红细胞样品的调制。从正常志愿者收集的红细胞中，元素图与红细胞形状相吻合，并倾向于形成小而致密的结节。血液透析患者的红细胞碎片比对照组更明显。血液透析患者体内的铁点倾向于部分聚集，并分成一个或两个室。慢性丙型肝炎患者接受干扰素治疗后，各成分分布较厚，分布于大多数红细胞，并呈明显的剂量依赖性。我们的研究表明，在空气中微量PIXE分析红细胞中的元素是有用的，以探讨与血液透析和干扰素治疗相关的贫血的发病机制。

项目成果

大気micro-PIXEを用いた透析患者の赤血球分析。

使用大气微型 PIXE 分析透析患者的红细胞。

• 发表时间: 2010
• 作者: 菊地弘記;時田佳治;長嶺竹明;佐藤隆博;神谷富裕
• 通讯作者: 神谷富裕

ヒト赤血球を用いた大気マイクロPIXE測定試料の作成

使用人类红细胞创建大气微 PIXE 测量样本

• 发表时间: 2009
• 作者: 時田佳治;菊地弘記;長嶺竹明;佐藤隆博;神谷富祐;荒川和夫
• 通讯作者: 荒川和夫

Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent

• DOI: 10.1016/j.cca.2010.09.040
• 发表时间: 2011-01-14
• 期刊: CLINICA CHIMICA ACTA
• 影响因子: 5
• 作者: Nakano, Takamitsu;Tokita, Yoshiharu;Nakajima, Katsuyuki
• 通讯作者: Nakajima, Katsuyuki

Attenuation of N-nitrosodiethylamine-induced liver fibrosis by high-molecular-weight fucoidan derived from Cladosiphon okamuranus

• DOI: 10.1111/j.1440-1746.2009.06187.x
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
• 影响因子: 4.1
• 作者: Nakazato, Kyoumi;Takada, Hisashi;Nagamine, Takeaki
• 通讯作者: Nagamine, Takeaki

Development of fucoidan-specific antibody and measurement of fucoidan in serum and urine by sandwich ELISA

岩藻依聚糖特异性抗体的开发以及夹心 ELISA 测定血清和尿液中的岩藻依聚糖

• 发表时间: 2010
• 期刊: Bioscience Biotechnology and Biochemistry
• 影响因子: 1.6
• 作者: Akiyama H;Kobayashi Z;Arai T;Hosokawa M;Tsuchiya K;Yokota O;Shimomura Y;Kondo H;Haga C;Hasegawa M;時田佳治
• 通讯作者: 時田佳治