Analysis of elemental distribution in erythrocytes using in-air-micro PIXE and its clinical application

贫血常与多种疾病相关，是化疗的不良反应。贫血并发抗癌药物、干扰素治疗和放射治疗日益严重，因此需要开发新的诊断技术工具。在这项研究中，我们建立了一种新的分析方法，即空气中微量元素在红细胞中的分布。首先，我们确定了红细胞样品在空气中进行微量pixe分析的最佳条件，确认了Ortega的改进方法适用于红细胞样品的调制。从正常志愿者身上采集的红细胞，元素图与红细胞形状相符，并趋向于形成致密的小结节。血透患者红细胞碎片明显多于对照组。血液透析患者表现为铁点有部分聚集的倾向，并分成一室或两室。在接受干扰素治疗的慢性丙型肝炎患者中，各元素分布较厚，分布在大多数红细胞中，这一发现明显呈剂量依赖性。我们的研究表明，空气中微量pixe分析红细胞中的元素有助于研究血液透析和干扰素治疗相关贫血的发病机制。

Anemia is often associated with various diseases and occurs as adverse effect of chemotherapy. Anemia complicated by anti-cancer drug, interferon therapy and radiation therapy becomes seriously, therefore development of new tool for diagnostic technique is desired. In this study, we established new analytical method of elemental distribution in erythrocytes using in-air mico-PIXE.At first, we determined the optimum condition of erythrocyte samples for in-air micro-PIXE analysis, confirming that the modified method of Ortega was suitable for modulation of erythrocyte samples. In erythrocytes collected form normal volunteers, elemental maps fit with the erythrocyte shape and tended to form small dense nodules. Erythrocyte fragments were more conspicuous in hemodialysis patients than controls. Hemodialysis patients showed that iron dots tended to aggregate partially and be divided into one or two compartments. In chronic hepatitis C patients received interferon therapy, each element distributed thickly and spread over most erythrocytes, this finding became apparently in dose dependent manner. Our study suggest that analysis of elements in erythrocytes by in-air micro-PIXE is useful to investigate pathogenesis of anemia associated with hemodialysis and interferon therapy.