The influence of ghrelin pormorplism on the occurence of cardiovascular disease and gastric acid secretion

我们于2004年4月至2006年3月调查了胃饥饿素多态性对心血管疾病危险因素和胃酸分泌的影响。在获得书面知情同意后，112例前来进行大规模筛查的患者被纳入本研究。目标多态性位点为Arg51Gln（外显子2中的G346A）、Leu72Met（外显子2中的C408A）和内含子1中的G274A。本研究112例受试者中均无G346A和G274A区域突变基因。在C408A区发现41例C408A异型突变，3例突变/突变。C408A区突变与幽门螺杆菌感染的关系未见观察。此外，C408A区域突变与血浆ghrelin浓度、血清总胆固醇、hdl -胆固醇和甘油三酯浓度无关。下一步我们将进一步研究C408A区多态性与胃酸分泌的关系。在我们进行这项研究期间，我们澄清了日本人群中非糜烂性胃食管反流病的患病率。阐明了动脉硬化程度与裂孔疝发生率的关系。

We investigated the influence the ghrelin pormorphism on the risk factors of cardiovascular diseases and gastric acid secretion between April 2004 and March 2006. After obtaining written informed consent,112 cases, who visited for mass screening, were enrolled in this study. Target polymorphic sites were Arg51Gln (G346A in exon-2), Leu72Met (C408A in exon-2) and G274A in intron-1. None of the 112 subjects had mutant gene in G346A and G274A regions in this study subjects. In C408A region, we found 41 subjects with hetero-type C408A mutation and 3 subjects with mutant/mutant. The relationship between the mutation of C408A region and Helicobacter pylori infection was not observed. In addition, the mutation of C408A region was not correlated with the plasma ghrelin concentration and serum concentrations of total cholesterol, HDL-cholesterol, and triglyceride. We are planning to investigate the relationship between the pormorphism of C408A region and gastric acid secretion in next step. We clarified the prevalence of the cases with non-erosive gastro-esophageal reflux disease in Japanese population during we performing this study. The relationship between the degree of arteriosclerosis and the prevalence of hiatal hernia was also clarified.