The relationship between Helicobacter pylori infection and polymorphism of immune-related gene

我们进行了一项病例对照研究，以阐明幽门螺杆菌（H。pylori）感染及免疫相关基因多态性。研究参与者是健康的日本人，他们每年都参加体检。本研究的所有受试者均获得了书面知情同意书，并获得了岛根大学伦理委员会的批准。我们选择了96名血清学H. pylori和年龄、性别匹配的96例H.幽门。从白色血细胞中提取基因组DNA，分析肿瘤坏死因子（TNF）-α、IL-10、CD 14启动子区（-308）和甘露糖结合凝集素（MBL）两个位点的基因组多态性。因此，我们在这项研究中调查的五个免疫相关基因的多态性的有意义的差异，没有观察到幽门螺杆菌血清阳性和血清阴性受试者。因此，我们认为遗传免疫机制的差异可能不影响H。pylori感染。在我们进行这项研究期间，我们阐明了长期的H。幽门螺杆菌感染降低血清高密度脂蛋白胆固醇水平。然而，动脉硬化的程度受年龄的影响，而不是由H。幽门螺杆菌感染。此外，血清同型半胱氨酸水平在H。pylori血清阳性和阴性个体。我们在英文医学杂志上报道了这些发现。

We performed a case-control study to clarify the relationship between the formation of Helicobacter pylori (H. pylori) infection and polymorphisms of immune-related gene from April 2001 to March 2004. Study participants were healthy Japanese, who attended for annual medical check-up. written informed consents were obtained by all subjects for this study, which was approved by ethical committee of Shimane University. we selected 96 subjects with seropositive of H. pylori and age-and sex-matched 96 subjects with seronegative of H. pylori. Genomic DNA was extracted from white blood cell and analyzed the genomic polymorphisms in promotor region (-308) of tumor necrosis factor (TNF)-α, IL-10, CD 14 and two sites of Mannose-binding lectin (MBL). As a result, meaningful differences in polymorphism of five immune-related genes, we investigated in this study, were not observed between H pylori-seropositive and seronegative subjects. Therefore, we concluded that the difference in hereditary immune mechanism may not influence the formation of H. pylori infect ion.During we performed this study, we clarified chat long-term H. pylori infect ion reduces serum level of high-density lipoprotein cholesterol. However, the degree of arteriosclerosis is influenced by aging and not by H. pylori infect ion. In addition, serum homocysteine level is not different between H. pylori-seropositive and negative individuals. We reported these findings in English medical journal.