Remodeling of skeletal muscle-molecular mechanisms of muscle fiber type switching

在本研究中，我们研究了肌纤维类型转换的分子机制，特别是慢速到快速的纤维转换。利用慢纤维或快纤维特异性基因的报告质粒，筛选肌纤维类型特异性基因表达的顺式元件。例如，肌红蛋白基因启动子区域必须包含慢纤维特异性顺式元件，而肌球蛋白重链2A基因启动子可能包含快纤维特异性顺式元件。我们进行了多次这样的实验，发现了几个顺式元件肌肉纤维类型特异性基因的表达。

In this study we investigated the molecular mechanisms of muscle fiber type switching, especially slow to fast fiber switching. Using reporter plasmids for slow or fast fiber specific genes, we screened the cis-element for muscle fiber type specific gene expression. For example, the myoglobin gene promoter region must contain the slow fiber specific cis-element(s), whereas the myosin heavy chain 2A gene promoter could contain the fast fiber specific cis-element. We conducted these experiments several times and found the several cis-elements muscle fiber type specific gene expression.