T cell epitope of GAD antigen in type 1 diabetes

1型糖尿病GAD抗原T细胞表位

• 批准号: 16590894
• 负责人: SHIMADA Akira
• 金额: $ 2.3万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590894/
• 关键词: Type 1 diabetes; GAD; T cell; 糖尿病; 免疫学; HLA; ペプチド

Typical type 1 diabetic patients were recruited after obtaining informed consent and approval of ethics committee of Keio Univ. School of Medicine. Among those patients, 33 year old type 1 diabetic patient with GAD antibody, short duration of disease, and HLA DR9 was selected. Peripheral blood lymphocytes were obtained from the patient, and those were stimulated with recombinant human GAD antigen to establish GAD reactive T cell clones. We could obtain a T cell clone, and confirmed that the phenotype of the clone is CD4, and indeed the clone reacts specifically to GAD antigen. We are now stimulating the clone with GAD peptide to identify the T cell epitopes.

在获得知情同意书和庆应义塾大学医学院伦理委员会批准后，招募典型的1型糖尿病患者。在这些患者中，选择具有GAD抗体、病程短和HLADR9的33岁1型糖尿病患者。从患者获得外周血淋巴细胞，并且用重组人GAD抗原刺激这些淋巴细胞以建立GAD反应性T细胞克隆。我们获得了一个T细胞克隆，并证实该克隆的表型为CD4，并且确实该克隆对GAD抗原具有特异性反应。我们现在用GAD肽刺激克隆以鉴定T细胞表位。

项目成果

GAD-reactive T cells were mainly detected in autoimmune-related type 1 diabetic patients with HLA DR9

GAD反应性T细胞主要在携带HLA DR9的自身免疫相关1型糖尿病患者中检测到

• 发表时间: 2004
• 期刊: Ann N Y Acad Sci. 1037
• 作者: 中崎満浩;加計正文;Itoh A et al.
• 通讯作者: Itoh A et al.

GAD-reactive T cells were mainly detected in autoimmune-related type 1 diabetic patients with HLA DR9.

GAD反应性T细胞主要在携带HLA DR9的自身免疫相关1型糖尿病患者中检测到。

• 期刊: Ann N Y Acad Sci. (In press)
• 作者: Arata Itoh;Akira Shimada
• 通讯作者: Akira Shimada