Study of new special immunosuppressive strategy for ABO incompatible transplantation

ABO血型不合移植特殊免疫抑制新策略的研究

• 批准号: 16591244
• 负责人: KAWAGISHI Naoki
• 金额: $ 2.24万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591244/
• 关键词: incompatible; liver transplantation; living; ELIZA; antibody titer; organ transplantation; immunosuppression; rejection; 肝臓移植; 腎臓移植

In Japan, the shortage of grafts has encouraged us to perform living donor liver transplantation (LDLT) across the ABO blood barrier. To decide the treatment for an ABO-I recipient, the amount of anti-A/B antibody (Ab) is very important. At present, the quantity of anti-A/B Ab is represented by the agglutination titer. We developed an enzyme linked immunosorbent assay (ELISA) to measure anti-A/B Ab, and assessed its usefulness in clinical practice in this study.In the assessment of all sera, the correlation between the O.D. and the titer of anti-A/B Ab were moderate. However, in the assessment of each recipient, the correlation between the O.D. and the titer of anti-A/B Ab was strong or moderate. Moreover, this investigation suggested that with our method it was possible to detect Ab-mediated rejection much earlier.In conclusion, our ELISA method is useful to detect changes in individually anti-A/B Ab and may have the possibility of using ELISA in clinical cases.

在日本，移植物的短缺鼓励我们进行跨越 ABO 血屏障的活体肝移植 (LDLT)。为了决定 ABO-I 受体的治疗，抗 A/B 抗体 (Ab) 的量非常重要。目前，抗A/B抗体的量以凝集滴​​度来表示。我们开发了一种酶联免疫吸附测定 (ELISA) 来测量抗 A/B 抗体，并在本研究中评估了其在临床实践中的有用性。抗A/B抗体效价中等。然而，在对每个接受者的评估中，O.D.之间的相关性。抗A/B抗体效价为强或中等。此外，这项研究表明，使用我们的方法可以更早地检测到 Ab 介导的排斥反应。 总之，我们的 ELISA 方法可用于检测个体抗 A/B Ab 的变化，并且可能有可能在临床病例中使用 ELISA。

项目成果

Enzyme linked immunosorbent assayによる抗血液型抗体の測定

酶联免疫吸附测定抗血型抗体

• 发表时间: 2006
• 期刊: 日本消化器外科学会雑誌 39巻1号
• 作者: 佐藤明史;川岸直樹;峯岸正好;土井秀之;里見進
• 通讯作者: 里見進

Measurement of antibody by enzyme linked immunosorbent assay

通过酶联免疫吸附测定法测定抗体

• 发表时间: 2006
• 期刊: Japanese Journal of Gastroenterological Surgery Vol.39
• 作者: Akefumi Sato;Naoki Kawagishi
• 通讯作者: Naoki Kawagishi