ElViS - Electrochemical Virus (Covid-19) Sensors based on Printed and Langmuir Blodgett assembled 2D materials; fast, cheap, reliable, point of care devices.

ElViS - 基于印刷和 Langmuir Blodgett 组装的 2D 材料的电化学病毒 (Covid-19) 传感器；

• 批准号: 471726913
• 负责人: Dr. Beata Maria Szydlowska, Ph.D.
• 金额: --
• 依托单位: Department of Materials Science and Engineering
• 依托单位国家: 德国
• 项目类别: WBP Fellowship
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/471726913?language=en
• 关键词: ElViS; Electrochemical; Virus; Covid; 19

The objective of this project is to develop an inexpensive (< Eur 3) point-of-service, self-administered testing platform for rapid (< 15 min) and accurate detection of SARS-CoV-2 from human saliva samples which will be simultaneously inclusive to the whole range of individuals in the global society. Initial stage of the project will be concerning development of ink suitable for Langmuir Blodgett (LB) and Aerosol Jet Printing (AJP) and characterized by long time stability in environmental conditions as well prolongated shelf life. The low cost is enabled by the use of Liquid Phase Exfoliated graphene / PtSe2 interdigitated electrodes (IDEs), rapidly prototyped by AJP and LB with careful consideration of size dependent properties of the material building the active platform. The high manufacturing throughput of both these techniques will be run in parallel. Next to determining best suited nanosheets size gan IDE geometry it will enable immediate, massive deployment of proposed sensing technology in addition to providing the possibility of multiple tests per patient, allowing for statistical analysis that will minimize false negatives or positives. The graphene IDEs will be functionalized with polyclonal and/or monoclonal antibodies that are highly specific to the S1 spike protein of the SARS-CoV-2 virion to form the biosensing platform. The high surface area of the printed graphene IDEs is expected to enable increased antibody loading to allow detection of low concentrations of SARS-CoV-2 (< 200 copies/mL) and amplify electrochemical signaling. The biosensor sensitivity, selectivity, and propensity for false negatives or positives will be evaluated by electrochemical impedance spectroscopy (EIS), < 15 min for incubation and scanning. Importantly, proposed sensors will require very low amounts of saliva (est. volume ~100 uL). That is strikingly beneficial in elderly patients, often suffering from dry mouth where collecting such an amount is not possible. The SARS-CoV-2 biosensing platform will be adapted into a form factor compatible with existing portable electrochemical potentiostats (e.g., glucometers used to read home glucose test strips) so that data can be easily acquired by the end user and transmitted, if desired, by Bluetooth to a laptop/smartphone for further on-site analysis. Importantly not only costs but the reliability of the sensor response is here of an utmost importance. Developed sensors must be characterized by low at best close to zero number of false positive and negative results. Ultimately, this platform will enable widespread and rapid home testing for individuals in quarantine or “gate check” testing to prevent infected individuals from entering crowded areas such as factories, airports and health care facilities. As a future outlook, the research effort of this project could help to quickly address new virus mutations and variants as well to other viruses making it extremally beneficial for the society.

该项目的目标是开发一种廉价（< 3欧元）的自助式检测平台，用于从人类唾液样本中快速（< 15分钟）准确检测SARS-CoV-2，该平台将同时涵盖全球社会中的所有个体。该项目的初始阶段将涉及开发适合Langmuir Blodgett （LB）和气溶胶喷射印刷（AJP）的油墨，其特点是在环境条件下具有长时间稳定性和延长的保质期。低成本是通过使用液相剥离石墨烯/ PtSe2交叉指状电极（IDEs）实现的，AJP和LB在仔细考虑了构建活性平台的材料的尺寸依赖特性的情况下快速原型。这两种技术的高制造吞吐量将并行运行。除了确定最适合纳米片尺寸的gan IDE几何形状外，它还可以立即大规模部署拟议的传感技术，并为每位患者提供多次测试的可能性，允许进行统计分析，以最大限度地减少假阴性或阳性。石墨烯ide将与SARS-CoV-2病毒粒子S1刺突蛋白高度特异性的多克隆和/或单克隆抗体功能化，形成生物传感平台。打印的石墨烯ide的高表面积有望增加抗体负载，从而检测低浓度的SARS-CoV-2（< 200拷贝/mL）并放大电化学信号。生物传感器的灵敏度、选择性和假阴性或假阳性倾向将通过电化学阻抗谱（EIS）进行评估，孵育和扫描时间小于15分钟。重要的是，拟议的传感器将需要非常低的唾液量（测试体积~100 uL）。这对老年患者非常有益，因为他们经常患有口干，不可能收集到这么多的水。SARS-CoV-2生物传感平台将被改造成与现有便携式电化学电位器（例如，用于读取家庭血糖试纸的血糖仪）兼容的外形，以便最终用户可以轻松获取数据，并在需要时通过蓝牙将数据传输到笔记本电脑/智能手机，以进行进一步的现场分析。重要的是，不仅成本，而且传感器响应的可靠性在这里是至关重要的。发达的传感器必须具有低（最好接近于零）假阳性和假阴性结果的特征。最终，该平台将能够对隔离人员进行广泛和快速的家庭检测或“门口检查”检测，以防止感染者进入工厂、机场和卫生保健设施等拥挤区域。展望未来，该项目的研究工作可以帮助快速解决新的病毒突变和变体以及其他病毒，使其对社会极为有益。