Brain protection during antegrade selective cerebral perfusion in patients with old cerebral infarction.

陈旧性脑梗死患者顺行选择性脑灌注时的脑保护作用

• 批准号: 16591391
• 负责人: WASHIYAMA Naoki
• 金额: $ 2.24万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591391/
• 关键词: Total arch replacement; Antegrade selective celebral perfision; Brain protection; Cerebral infarction; Edaravone; SEP; Hexanoyl-lysine; Malondialdehyde; Cerebral protection; Hexanol-lysine; MDA; 8-OHdG

Objective : Reactive free radical species are thought to be involved in postoperative neurological dysfunction after antegrade selective cerebral perfusion (ASCP) in brain with old infarction. We assessed the brain protective effect of prophylactically administered edaravone, a free radical scavenger, for ASCP in brains with or without old infarction in a canine model.Methods : Canine model of old cerebral infarction was created by injecting cylindrical silicone embolus into the middle cerebral artery. Animals showing obvious neurologic deficits and surviving 4 weeks or longer were included in the model. Deep hypothermia with ASCP was performed in both intact (non-edaravone; group A, edaravone-treated; group B) and infarcted animals (non-edaravone; group C, edaravone-treated; group D). Serum concentrations of malondialdehyde (MDA), hexanoyle-lysine (HEL), glutamate, and venous-arterial lactate difference (VALD) were measured and central conduction time (CCT) and amplitude of somatosensory evoked potentials were assessed during the operation.Results : Compared with the intact groups, serum concentrations of MDA and HEL in group C significantly increased at the end of ASCP, while that of glutamate did so in the rewarming phase. Increase in all these biochemical parameters were suppressed in group D. In group C, VALD was significantly greater in the rewarming phase at 28℃ compared with intact groups. Significant prolongation of post operative CCT and decrease in neuronal activity were detected in group C, both of which recovered in group D.Conclusion : Prophylactic administration of edaravone exerted a significant protective effect against postoperative neurological dysfunction after ASCP in a canine model with old cerebral infarction.

目的：反应性自由基被认为参与了陈旧性脑梗死顺行选择性脑灌注（ASCP）术后神经功能障碍。我们评估的脑保护作用的依达拉奉，自由基清除剂，ASCP在大脑中有或没有陈旧性梗死在犬model.Methods：犬陈旧性脑梗死模型是通过注射圆柱形硅胶栓塞到大脑中动脉。模型中包括表现出明显神经功能缺损且存活4周或更长时间的动物。在完整动物（非依达拉奉; A组，依达拉奉治疗; B组）和梗死动物（非依达拉奉; C组，依达拉奉治疗; D组）中进行ASCP深低温。术中检测血清丙二醛（MDA）、己酰赖氨酸（HEL）、谷氨酸（Glu）、静脉-动脉乳酸差（VALD），并测定中枢传导时间（CCT）和体感诱发电位（SEP）波幅。与正常对照组相比，C组血清MDA和HEL浓度在ASCP结束时显著升高，而谷氨酸则在复温阶段起作用。D组上述生化指标的升高均受到抑制。C组在28℃复温期的VALD显著高于未复温组。结论：依达拉奉预防性给药对陈旧性脑梗死犬ASCP术后神经功能障碍具有明显的保护作用。

项目成果

Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction

预防性给予依达拉奉对陈旧性脑梗死犬模型顺行脑灌注的影响

• 发表时间: 2007
• 期刊: J Thorac Cardiovasc Surg 133
• 作者: Suzuki T;Kazui T;Yamamoto S;Washiyama N;Ohkura K;Ohishi K;Bashar AH;Yamashita K;Terada H;Suzuki K;Akuzawa S;Fujie M
• 通讯作者: Fujie M