Study for epileptogenesis arising from the neuronal networks among amygdala, entorhinal cortex and hippocampus.

近年来，内嗅皮质的变性在边缘系统癫痫的发生中起着重要作用。我们用包括保持神经元联系的内嗅皮层的海马脑片研究了这一区域。到目前为止，我们的前一个项目已经阐明了内嗅皮质的癫痫发生机制。在本项目中，我们研究了抗癫痫药物对联合海马脑片记录的癫痫放电的抑制作用，并在水浴中应用喹诺酮和氟比洛芬（分别为抗菌药物和镇痛药物）。卡马西平的抑制作用最强，苯巴比妥次之，丙戊酸最弱。在四分之一的这些合并的海马旁切片没有任何药理学操作的周期性振荡活动。将神经网络保存在脑片中，通过手术分离海马旁神经元网络，发现海马和海马旁神经元网络的失同步活动，从而为分析边缘系统癫痫发作机制提供了有用的工具。

Recently, degeneration of entorhinal cortex, which fibers mostly connect to hippocampus proper, are playing an important role of epileptogenesis in limbic seizure. We have investigated this area with combined hippocampal slice which includes entorhinal cortices keeping neuronal connection. Thus far, the epileptogenesis of entorhinal cortices was elucidated by our former project.In this project, we investigated the inhibitory effect of antiepileptic drugs on epileptic discharges recorded from the combined hippocampal slices with bath application of Quinolone and Flurbiprofen (antibacterial drug and analgesic respectively). The strongest inhibition was shown by Carbamazepine, followed by Phenobarbital and Valproic Acid.We also developed combined parahippocampal slice including amygdala. Periodic oscillatory activity was identified in a quarter of these combined parahippocampal slices without any pharmacological manipulation. The neural networks were kept in these slices, identifying with de-synchronization of this activity after surgical separation of these slices to disconnect the parahippocampal neuronal networks.Teses combined hippocampal and parahippocampal slices are useful tools for analyzing the underlying mechanisms of limbic seizure.