Study for epileptogenesis arising from the neuronal networks among amygdala, entorhinal cortex and hippocampus.

研究杏仁核、内嗅皮层和海马之间的神经网络引起的癫痫发生。

• 批准号: 16591462
• 负责人: NAGAO Takeki
• 金额: $ 1.6万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591462/
• 关键词: slice; epilepsy; hippocampus; entorhinal cortex; amygdala; antiepileptic drug; 切片

Recently, degeneration of entorhinal cortex, which fibers mostly connect to hippocampus proper, are playing an important role of epileptogenesis in limbic seizure. We have investigated this area with combined hippocampal slice which includes entorhinal cortices keeping neuronal connection. Thus far, the epileptogenesis of entorhinal cortices was elucidated by our former project.In this project, we investigated the inhibitory effect of antiepileptic drugs on epileptic discharges recorded from the combined hippocampal slices with bath application of Quinolone and Flurbiprofen (antibacterial drug and analgesic respectively). The strongest inhibition was shown by Carbamazepine, followed by Phenobarbital and Valproic Acid.We also developed combined parahippocampal slice including amygdala. Periodic oscillatory activity was identified in a quarter of these combined parahippocampal slices without any pharmacological manipulation. The neural networks were kept in these slices, identifying with de-synchronization of this activity after surgical separation of these slices to disconnect the parahippocampal neuronal networks.Teses combined hippocampal and parahippocampal slices are useful tools for analyzing the underlying mechanisms of limbic seizure.

近年来，内嗅皮层的退行性变在边缘癫痫发作中起着重要的作用。我们用联合海马体切片研究了这一区域，其中包括保持神经元连接的内嗅皮层。到目前为止，内嗅皮层的癫痫发生是由我们之前的项目阐明的。在本项目中，我们研究了抗癫痫药物对海马片记录的癫痫放电的抑制作用，并分别应用喹诺酮和氟比洛芬（抗菌药物和镇痛药物）。卡马西平的抑制作用最强，其次是苯巴比妥和丙戊酸。我们还开发了包括杏仁核在内的联合海马旁切片。在没有任何药理学操作的情况下，在这些联合海马旁切片的四分之一中发现了周期性振荡活动。神经网络保留在这些切片中，在手术分离这些切片以断开海马旁神经元网络后，识别出这种活动的去同步化。结合海马和海马旁切片是分析边缘癫痫潜在机制的有用工具。