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Expression and the role of nuclear matrix protein HMGI (Y) in human renal cell carcinoma

核基质蛋白HMGI(Y)在人肾细胞癌中的表达及作用

基本信息

批准号：
16591594
负责人：
NONOMURA Norio
金额：
$ 2.24万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

To examine the specificity of the antibody against human HMGI(Y), we performed immunohistochemical staining of HMGI(Y) using mouse xenograft model of human prostatic cancer. In Western blot analysis, HMGI(Y) was detected using nuclear protein extracted from PC3 cells. Although HMGI(Y) is a nuclear matrix protein, it was stained in nucleus and also in cytosol. Therefore, immunohistochemical approach was not thought to be appropriate to examine the expression of HMGI(Y) in surgical resected specimens. On the other hand, polymerase chain reaction showed 164 base pair band corresponding to PCR product of HMGI(Y) in all renal cancer cell lines (NC65, ACHN, OUR10, Caki-l). Then, we examined real-time PCR for quantify HMGI(Y) expression in these renal cancer cell lines. This method also detected its expression in freshly-frozen tissues which were surgically resected. Interestingly, the expression of HMGI(Y) in cancer tissues was more abundant than in normal renal tissues. Moreover, its expression in cancer tissues was higher than in normal tissues from the same patients. In cancer tissues from patients with T2-3 tumor HMGI(Y) expressed more intensively than in cancer tissues of T1 tumor. The possibility for prognostic factor of the expression of HMGI(Y) in renal cancer was examined. Patients with high expression of HMGI(Y) tend to live longer than those with low expression.Therefore, the expression of HMGI(Y) in renal cell carcinoma may be a possible candidate as a prognostic factor.

为了检测抗人HMGI（Y）抗体的特异性，我们使用人前列腺癌的小鼠异种移植模型进行HMGI（Y）的免疫组织化学染色。在Western印迹分析中，使用从PC 3细胞提取的核蛋白检测HMGI（Y）。虽然HMGI（Y）是一种核基质蛋白，但它在细胞核和细胞质中也有染色。因此，免疫组织化学方法被认为不适合于检测手术切除标本中HMGI（Y）的表达。另一方面，聚合酶链反应显示在所有肾癌细胞系（NC 65、ACHN、OUR 10、Caki-1）中对应于HMGI（Y）的PCR产物的164个碱基对条带。然后，我们检测了实时PCR定量HMGI（Y）在这些肾癌细胞系中的表达。该方法还检测了其在手术切除的新鲜冷冻组织中的表达。有趣的是，HMGI（Y）在癌组织中的表达比在正常肾组织中更丰富。此外，其在癌组织中的表达高于来自相同患者的正常组织。在T2-3期肿瘤组织中HMGI（Y）的表达强度高于T1期肿瘤组织。探讨HMGI（Y）表达作为肾癌预后因素的可能性。HMGI（Y）高表达者的生存期较低表达者长，因此HMGI（Y）在肾癌中的表达可能是一个预后指标。