Analysis on the polymorphism of genotype of enzymes for detoxication in patients with infertility

复发性流产的病因尚未完全阐明，解毒酶基因型与复发性流产之间的关系最近被注意到。因此，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析反复流产的不孕症患者解毒酶基因型，如细胞色素P450 （CYPIAI）、谷胱甘肽- s -转移酶pai （GST-pai）、GST-my、GST-theta。研究人员向患者发放了有关吸烟史或饮酒史的问卷。到目前为止，共有44名患者加入了这项研究，另外还有30名从未经历过堕胎的正常生育妇女也加入了这项研究。比较患者群体和对照组各基因型酶的频率。关于CYPIAI的没有发现显著差异,glutathione-S-transferase-pai (GST-pai),销售税。然而，GST-pai的la基因型频率在患者组明显高于对照组。为了得到明确的结论，必须进一步调查，增加患者和对照人群的数量

The etiology of the recurrent miscarriage has not been fully elucidated, and the relationship between the genotype of enzymes for detoxication and recurrent miscarrage has recently been noted. Thus, the genotypes of enzymes for detoxication, such as cytchrome P450 (CYPIAI), glutathione-S-transferase-pai (GST-pai), GST-my, GST-theta, were analyzed in patients with infertility, who had experienced recurrent miscarriages by means of polymerase-chain reaction- restrication fragment length polymorphism (PCR-RFLP) method. The patients were sent out the questionnaires concernig history of smoking, or alchol intake. So far, 44 patients were enrolled into the study, and 30 normal fertile women, who had never experienced abortions, were also enrolled into this study.The frequency of each genotype of the enzymes was compared between the patient population and control group. The significant difference was not observed concerning CYPIAI, glutathione-S-transferase-pai (GST-pai), GST my. However, the frequency of la genotype of GST-pai was signinficantly higher in patients group compared with control population. In order to obtain definite conclusion, further investigation is considered to be mandatory, increasing the number of the patients and control population