Development of the new embryo transfer method which paid attention to embryo factors

两步胚胎移植的有效性调查：在滋贺大学医学部妇产科及其相关医院进行了人类体外受精和胚胎移植。通过生育率、卵裂数、平均移植胚胎数、妊娠率、种植率、流产率、新生儿畸形发生率、多胎率、卵巢过度刺激综合征（OHSS）发生率、患者年龄和体外受精尝试次数等多项参数研究临床有效性。比较两步胚胎移植和一步胚胎移植的效果。处理程序如下：1）从前一排卵周期的黄体期第7天开始给予GnRH激动剂600 mg/d，2）从排卵前11天开始每天注射225单位的纯FSH，3）在排卵前11天收集卵泡， 关于我们 4）在含有10%合成血清替代物（SSS）的P1培养基中进行授精和胚胎培养; 5）在受精后第2天和第5天进行经腹超声引导的胚胎移植。2005年3月，部分临床结果发表在美国《生育与不育》杂志上。本研究由Koichi Takebayashi，M.D和Fuminori Kimura，M. D进行，目的是为了进一步提高统计学的可靠性。尚未鉴定出胚形成因子。然而，作为使用小鼠模型的实验研究，研究了一组基因，其基因表达由胚胎依赖性子宫内膜诱导。部分结果在2004年日本妇产科学会第56届年会上报告。少

Investigation into the effectiveness of the two step embryo transfer :Human in vitro fertilization with embryo transfer has been conducted at the Obstetric and Gynecologic Department of Shiga University School of Medicine and its related hospitals. The clinical effectiveness is investigated with various parameters including the fertility rate, number of cleavages, average number of embryos transferred, pregnancy rate, implantation rate, miscarriage rate, the incidence of neonatal abnormality, multiplets rate, the incidence of ovarian hyperstimulation syndrome (OHSS), age of patients, and the number of in vitro fertilization attempted. The results of the two-step embryo transfer and one-step embryo transfer were compared. The management procedure was as follows : 1) GnRH agonists were administered 600 mg/day from day 7 of the luteal phase of the previous ovulation cycle, 2) pure FSH was injected every day at 225 units from 11 days before ovulation, 3) ovarian follicles were collected on … More the ovulation day after confirming maturation of the follicles, 4) insemination and embryo culture were done in P1 medium containing 10% synthetic serum substitute (SSS), and 5) transabdominal ultrasound-guided embryo transfer was done on day 2 and day 5 after fertilization. Part of the clinical results were published in the American journal "Fertility and Sterility" in March, 2005. This study has been ongoing with an increasing number of parameters to improve statistical reliability.Investigation into blastogenic endometrial modifying factors and embryo implantation induction substances :This study has been conducted by Koichi Takebayashi, M.D, and Fuminori Kimura, M.D. Identification of blastogenic factors has not yet been achieved. However, as an experimental study using a mouse model, a group of genes was investigated, for which the gene expression was induced by the embryo-dependent endometrium. Part of the results were reported at the 56th Annual Congress of the Japan Society of Obstetrics and Gynecology in 2004. Less