Development of the new embryo transfer method which paid attention to embryo factors

关注胚胎因素的新型胚胎移植方法的开发

• 批准号: 16591655
• 负责人: NODA Yoichi
• 金额: $ 2.37万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591655/
• 关键词: Embryonic factor(s); Two step embryonic transfer; IVF-ET; 2段階胚移植

Investigation into the effectiveness of the two step embryo transfer :Human in vitro fertilization with embryo transfer has been conducted at the Obstetric and Gynecologic Department of Shiga University School of Medicine and its related hospitals. The clinical effectiveness is investigated with various parameters including the fertility rate, number of cleavages, average number of embryos transferred, pregnancy rate, implantation rate, miscarriage rate, the incidence of neonatal abnormality, multiplets rate, the incidence of ovarian hyperstimulation syndrome (OHSS), age of patients, and the number of in vitro fertilization attempted. The results of the two-step embryo transfer and one-step embryo transfer were compared. The management procedure was as follows : 1) GnRH agonists were administered 600 mg/day from day 7 of the luteal phase of the previous ovulation cycle, 2) pure FSH was injected every day at 225 units from 11 days before ovulation, 3) ovarian follicles were collected on … More the ovulation day after confirming maturation of the follicles, 4) insemination and embryo culture were done in P1 medium containing 10% synthetic serum substitute (SSS), and 5) transabdominal ultrasound-guided embryo transfer was done on day 2 and day 5 after fertilization. Part of the clinical results were published in the American journal "Fertility and Sterility" in March, 2005. This study has been ongoing with an increasing number of parameters to improve statistical reliability.Investigation into blastogenic endometrial modifying factors and embryo implantation induction substances :This study has been conducted by Koichi Takebayashi, M.D, and Fuminori Kimura, M.D. Identification of blastogenic factors has not yet been achieved. However, as an experimental study using a mouse model, a group of genes was investigated, for which the gene expression was induced by the embryo-dependent endometrium. Part of the results were reported at the 56th Annual Congress of the Japan Society of Obstetrics and Gynecology in 2004. Less

二步胚胎移植效果的研究：志贺大学医学院妇产科及相关医院开展了人类体外受精-胚胎移植的研究。观察受精率、卵裂数、平均移植胚胎数、妊娠率、种植率、流产率、新生儿畸形发生率、多胎率、卵巢过度刺激综合征(OHSS)发生率、患者年龄、体外受精次数。比较两步胚胎移植和一步胚胎移植的效果。处理程序如下：1)从前一个排卵周期的黄体期第7天开始每天注射促性腺激素释放激素激动剂6 0 0 mg；2)从排卵前11天开始每天注射2 2 5个单位的纯卵泡刺激素；3)在…上收集卵泡在确认卵泡成熟后的排卵当天，4)在含有10%合成血清替代品(SSS)的P1培养液中进行授精和胚胎培养，5)在受精后第2天和第5天进行经腹超声引导胚胎移植。部分临床研究结果于2005年3月发表在美国期刊《生育与不育》上。这项研究一直在进行中，为了提高统计可靠性，参数越来越多。胚胎性子宫内膜修饰因子和胚胎着床诱导物质的调查：这项研究是由Koichi Takebayashi医学博士和Finoori Kimura医学博士进行的，但对胚泡形成因素的鉴定尚未实现。然而，作为一项使用小鼠模型的实验研究，研究了一组基因，这些基因的表达是由胚胎依赖的子宫内膜诱导的。部分研究结果在2004年日本妇产科学会第56届年会上发表。较少

项目成果

ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles.

• DOI: 10.1083/jcb.200411179
• 发表时间: 2005-03-14
• 期刊: The Journal of cell biology
• 作者: Shimizu Y;Thumkeo D;Keel J;Ishizaki T;Oshima H;Oshima M;Noda Y;Matsumura F;Taketo MM;Narumiya S
• 通讯作者: Narumiya S

A case of quadruplet pregnancy with a complete hydatidiform mole

四胞胎妊娠合并完全性葡萄胎一例

• 发表时间: 2005
• 期刊: Prenat Diagon 25
• 作者: T.Yokoo;K.Takakuwa;et al.;Amano Tsukuru
• 通讯作者: Amano Tsukuru

生命の誕生にむけて 生殖補助医療(ART)胚培養の理論と実際

辅助生殖技术（ART）胚胎培养走向生命诞生的理论与实践

• 发表时间: 2005
• 作者: 竹林浩一;藤原睦子
• 通讯作者: 藤原睦子

Effectiveness of two-step (consecutive) embryo transfer in patients who have two embryos on day 2: comparison with cleavage-stage embryo transfer

• DOI: 10.1016/j.fertnstert.2004.07.974
• 发表时间: 2005-03-01
• 期刊: FERTILITY AND STERILITY
• 影响因子: 6.7
• 作者: Goto, S;Shiotani, M;Noda, Y
• 通讯作者: Noda, Y

A case of quadruplet pregnancy with a complete hydatidiformmole

完全性葡萄胎四胞胎妊娠一例

• 发表时间: 2005
• 期刊: Prenat Diagon 25
• 作者: 宮澤克人;鈴木孝治;Amano Tsukuru
• 通讯作者: Amano Tsukuru