Possible role of stathmin in the uterine function and in the mechanism of endometrial stroma decidualization

研究了安定素及其家族基因在小鼠子宫着床期的表达情况。妊娠早期，安定素主要存在于腺腔上皮、血管和子宫内膜的基质细胞中。安定素的强烈表达仅限于毛细血管和次级蜕细胞。Stathmin在着床部位的表达高于着床部位之间的子宫段，在油诱导的脱个体化过程中表达增加。抑素- ko小鼠的新生幼崽较少（减少30%）。碱性磷酸酶、desmin和细胞周期蛋白D3在stathmin-KO小鼠的各个区表达减弱。镇静素家族基因SCG10的信使RNA水平在WT和镇静素- ko小鼠去个化时较高。相反，与WT小鼠相比，SCLIP和RB3在stathmin-KO小鼠中高表达。这些结果表明，安定素和安定素家族基因在小鼠子宫内膜中表达，并在去个体化过程中表达增强。由于stathmin在人子宫中的表达尚未被研究，因此stathmin在人子宫中的功能被执行。Stathmin表达于人子宫内膜组织的腺上皮和基质细胞，以及妊娠早期的细胞滋养层细胞和上皮外滋养层细胞。当从正常子宫内膜分离的间质细胞被卵巢类固醇或环二丁基AMP刺激去个体化后，磷酸化的安定素水平逐渐下降，提示安定素可能参与子宫内膜间质细胞的去个体化。然而，在细胞暴露于脱醛剂之前，用siRNA敲低stastmin在培养的基质细胞中的表达，可以显著抑制脱醛，表现为igf结合蛋白-1和催乳素的表达降低。安定素在人子宫内膜和胎盘细胞中的表达不同，可能参与子宫内膜间质细胞的去个体化。少

The expression of stathmin and its family genes was examined in the murine uterus during the peri-implantation period. During the early stage of pregnancy, stathmin was mainly localized in the glandular and luminal epithelium, blood vessels, and stromal cells in the endometrium. Intense stathmin expression was limited to capillary vessels and secondary decidual cells. Stathmin expression was higher at implantation sites than at uterine segments between implantation sites and increased during oil-induced decidualization. The stathmin-KO mice had fewer newborn pups (reduced by 30%). The expression of alkaline phosphatase, desmin, and cyclin D3 was attenuated in decidual zones of stathmin-KO mice. Messenger RNA level of the stathmin family gene, SCG10, was high at the time of decidualization in WT and stathmin-KO mice.In contrast, SCLIP and RB3 were highly expressed in stathmin-KO mice compared to WT mice. These results suggest that stathmin and stathmin family genes are expressed in the … More murine endometrium with enhanced expression in the decidualization process. Because stathmin expression in the human uterus has not yet been investigated, the function of stathmin in human uterus was executed. Stathmin is expressed in the glandular epithelium and stromal cells of human endometrial tissue, and in cytotrophoblasts and extravillous trophoblasts during the first-trimester of pregnancy. When stromal cells isolated from normal endometrium were cultured and stimulated to decidualize by ovarian steroids or dibutyryl cyclic AMP, phosphorylated stathmin levels gradually decreased, suggesting stathmin may participate in endometrial stromal cell decidualization. However, knocking down stathmin expression in the cultured stromal cells by using siRNA before the cells were exposed to the decidualizing agents significantly suppressed decidualization, as indicated by the decreased expression of IGF-binding protein-1 and prolactin. Stathmin is differently expressed in human endometrial and placental cells and may be involved in the decidualization of endometrial stromal cells. Less