Establishment of hybrid type artificial bone using vascularized periosteal flap and PHAB

为了阐明实验性骨膜附着羟基磷灰石块成骨过程中孔隙的时序性变化，对兔模型进行了详细的显微镜观察和孔隙形态计量学分析。在整个实验过程中，对照组的PHAB嵌在肌肉中的孔中既没有发现骨也没有发现脂肪组织。与此相反，新形成的骨组织证实了层状结构，并且在第8周时，在矩形PHAB的骨膜覆盖的面部附近存在的孔隙的边缘处最初发现去极化，并且这种孔隙中的骨形成从覆盖的面部延伸到远端部分。第12周时，骨孔内可见脂肪组织，骨孔内有大量脂肪脱落。形态计量学分析显示，每个孔中骨面积百分比与PHAB附着骨膜面的距离呈负相关，推测附着于PHAB表面的骨膜提供的间充质干细胞分化诱导了骨脱落形成，这些骨脱落形成内衬于孔中并由脂肪组织填充，并有成红细胞胰岛。我们希望强调的是，PHAB附着带血管骨膜的复合物将成为体内骨重建的良好和安全的材料之一。

To elucidate the sequential change of experimental osteogenesis in pores of periosteum-attached hydroxyapatite blocks, detailed microscopic observations and morphometric analysis of pores were carried out on the rabbit model. Neither bone nor adipose tissue was found in the pores of PHAB embedded in the muscle during the entire experimental period in the control group. By the contrast, newly formed bone tissue confirmed laminated architecture and depolarization was initially found at the margin of the pores present close to the face covered by periosteum of rectangular-shaped PHAB at 8th week, and this bone formation in pores extended to distal portion from the covered face. On the 12th week, adipose tissue was observed inside of the bone sloughing lined the pore. The morphometric analysis revealed a negative correlation between the percentage in area of bone in each pore and the distance from the face of PHAB attached periosteum.The result surmised that bone-sloughing formation lining pores and filled by adipose tissue with islets of erythroblasts is induced by differentiation of mesenchymal stem cell provided via periosteum attaching onto a face of PHAB. We wish to emphasize that complex of PHAB attached vascularized periosteum presented would become one of the favorable and secure material for bone reconstruction in vivo.