权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mechanisms of drug-resistance and apoptosis in oral carcinoma cells

口腔癌细胞耐药及凋亡机制

基本信息

批准号：
16591893
负责人：
OHBA Takeshi
金额：
$ 2.18万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591893/
关键词：
drug-resistance cells transcriptional factor nuclear proteins apoptosis nucleorin okadaic acid cancer cells protein dephosphorylation 核内蛋白ニュークレオフォスミン AgNORs 制癌剤口腔癌細胞 RNAi 蛋白質脱リン酸化酵素

项目摘要

We examined the effects of anticancer drugs on induction of apoptosis and the transcriptional regulation of the multidrug resistance-1 (MDR1) gene in' oral squamous carcinoma cells. SCCKN cells were shown to be highly sensitive to various anticancer drugs, whereas SCCTF cells were minimally sensitive to these reagents. Peplomycin induced apoptosis in both SCCKN and SCCTF Cells in a dose-dependent fashion with the maximal effect at concentrations of 1 μM and 10 μM, respectively. Apoptosis was induced in SCCKN cells treated with 1 μM peplomycin, but not in SCCTF cells. RT-PCR revealed that MDR1 mRNA was highly expressed in SCCTF cells while it was under the limit of detection in SCCKN cells. After treatment with Hoechst 33342 to assess efflux action of MDR1 protein, intense staining occurred in SCCKN cells, whereas the staining was very weak in SCCTF cells. With an electrophoretic mobility shift assay using the MDR1 promoter region, a DNA-protein. Protein phosphorylation and dephosphorylation has been recognized as a key mechanism in cell function. Nucleolin is an abundantly expressed nucleolar phosphoprotein and is located mainly in the nucleolus. The staining pattern of nucleolin in cultured cells is similar to that of PP 1δ. Nucleolin was demonstrated to bind to PP1δ in nucleolus by using immunocytochemical and immunoprecipitation. methods. A major band, 110 kDa, was detected in the proteins obtained from the control cells. The level of the 110 kDa protein decreased in the apoptotic cells, whereas an additional band, 80 kDa, appeared and the level of this protein increased in the proteins prepared from okadaic acid-induced apoptotic cells. Our results indicate that PP1δ directly binds to nucleolin in the nucleolus and that nucleolin is cleaved during apoptosis.

我们研究了抗癌药物对口腔鳞癌细胞凋亡诱导和多药耐药基因1（MDR 1）转录调控的影响。SCCKN细胞对各种抗癌药物高度敏感，而SCCTF细胞对这些试剂的敏感性最低。培洛霉素以剂量依赖性方式诱导SCCKN和SCCTF细胞凋亡，最大效应分别为1 μM和10 μM。1 μM培洛霉素可诱导SCCKN细胞凋亡，但对SCCTF细胞无明显影响。RT-PCR结果显示，MDR 1 mRNA在SCCTF细胞中呈高表达，而在SCCKN细胞中则低于检测限。在用Hoechst 33342处理以评估MDR 1蛋白的外排作用后，SCCKN细胞中出现强染色，而SCCTF细胞中的染色非常弱。使用MDR 1启动子区的电泳迁移率变化测定，蛋白质磷酸化和去磷酸化已被认为是细胞功能的关键机制。核仁素是一种高表达的核仁磷蛋白，主要定位于核仁中。核仁素在培养细胞中的染色模式与PP 1δ相似。用免疫细胞化学和免疫沉淀法证明核仁素与核仁中的PP 1 δ结合。方法.在从对照细胞获得的蛋白质中检测到110 kDa的主带。110 kDa的蛋白质的水平下降的凋亡细胞，而一个额外的带，80 kDa，出现和该蛋白质的水平增加的蛋白质制备的冈田酸诱导的凋亡细胞。我们的研究结果表明，PP 1 δ直接结合核仁中的核仁素，核仁素在凋亡过程中被切割。

项目成果

期刊论文数量（66）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth

下颌骨髁皮质骨形成与生长过程中磁共振信号特征变化的关系

DOI：
发表时间：
2007
期刊：
Am J Orthod Dentofacial Orthop 131
影响因子：
0
作者：
Morimoto;Y.
通讯作者：
Y.

Cleavage of nucleolin and AgNOR proteins during apoptosis induced by anti-cancer drugs in human salivary gland cells.

抗癌药物诱导人唾液腺细胞凋亡过程中核仁素和 AgNOR 蛋白的裂解。

DOI：
发表时间：
2005
期刊：
Journal of Oral Pathology & Medicine 34
影响因子：
0
作者：
Ryoma Nakao;Hidenobu Senpuku;Haruo Watanabe;Tanaka T.;Inoue H;Morimoto Y;Ono K;Okabe S;Kito S;Kito S.;Morimoto Y.;Tanaka T.;Morimoto Y.;Tanaka T.;Kito S.
通讯作者：
Kito S.

Clinical application of magnetic resonance sialographic 3-dimensional reconstruction imaging and magnetic resonance virtual endoscopy for salivary gland duct analysis