Callus formation of membrous bone reconstruction using micro vascular endochondral bone

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Recently, plate-rich plasma (RPR) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of RPR from small quantities of blood.Various conditions were used fir anticoagulants and centrifugal separation speed for blood sample obtained from 23 healthy subjects to investigate optimal concentration condition. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r.p.m and 1300 r.p.m. The effective of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after centrifugation.