Investigation on the condition to achieve the best peripheral tissue reconstruction after maxillary alveolar distraction osteogenesis

为了探讨牵张成骨后获得最佳周围组织重建的条件，我们尝试提取1。新产生的愈伤组织和2.附近的牙齿进入的胼胝。在我们的初步研究中发现，血流量需要很长时间才能恢复到原来的水平，骨密度（BMD）甚至在牵引开始后100天也没有恢复到原来的水平。因此，我们看到了高压氧治疗（HBO）的优势，它已在临床上用于促进骨折的恢复，并旨在研究HBO对血流量和BMD的恢复使用比格犬的影响。在6只成年Beagle犬左侧腭部造成10 mm骨缺损，然后制成含犬的骨段，以1 mm/d的速度连续10 d。延长后，HBO管理三只狗。应用激光多普勒血流仪连续100天监测犬牙髓血流。处死后，解剖每个腭，并使用pQCT（外周定量计算机断层扫描）测量BMD。采用Mann-Whitney秩和检验对血流量和骨密度进行统计学分析。血流量恢复到原来的水平快30天，皮质骨密度高HBO治疗的狗。本研究提示高压氧对促进DOG的愈合有一定作用。关于牙齿向骨痂内移动的研究，我们刚刚建立了牙齿向骨痂内移动的实验系统。

To investigate the condition to achieve the best peripheral tissue reconstruction after distraction osteogenesis, we tried to extract the characteristics of 1. newly generated callus and 2. the callus after which nearby tooth has moved into. In our preliminary research, it is revealed that a long time was necessary for blood flow to recover to the original level, and the bone mineral density (BMD) did not get back to the original level even 100 days after distraction was initiated. Therefore, we saw the advantage of Hyperbaric Oxygen Therapy (HBO), which has been clinically used to facilitate the recovery of fractured bones, and aimed to investigate the influence of HBO on the recovery of blood flow and BMD using beagle dogs. The 10 mm bone defect was made in the left palate of 6 adult beagle dogs, then a bony segment including canine was made and transported at a rate of 1mm/day for 10 days. After the lengthening, HBO was administered to three dogs. Using laser Doppler flowmeter, blood flow of the canine pulp was monitored for 100 days. After the sacrifice, each palate was dissected and measured for BMD using pQCT (peripheral quantitative computed tomography). The blood flow and BMD were statistically analyzed using Mann-Whitney rank sum test. Blood flow recovered to the original level 30 days faster, the cortical BMD was higher in HBO-treated dogs. This study suggests that HBO is useful to facilitate the healing in DOG. In regard to the research on tooth movement into the generated callus, we have just established the experimental system of it.