Free radical and brain aging : role of metallothionein in the aging of Beagle dog I

自由基与脑衰老：金属硫蛋白在比格犬衰老中的作用 I

• 批准号: 10660300
• 负责人: SHIMADA Akinori
• 金额: $ 2.56万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660300/
• 关键词: Dog; Metallothionein; brain; aging; metal; iron; in situ hybridization; メタロチオフェイン; ノーザン ブロッティング; In situ ハイブリダイゼーション

Beagle dogs showed a variety of morphological age-related brain changes including neurona dystrophic axonal changes, astrogliosis and amyloidosis characterized by the formation of s plaques in the cerebral cortex. To study the roles of metallothioneins (MTs) in the brain, the expression pattern of metallothioneins in the brain of aged Beagle dogs was examined. Inter MT-I & -II immunoreactivity as well as signals for MT-I & -II mRNA were shown in the hypertrophic astrocytes in the brains with severe age-related changes. These MT-I & -II positive astrocytes dominated in the thalamus, cerebral cortex and medulla, where remarkal age-related changes including iron and lipofucin deposition were observed. In contrast, MT-I II expression was considerably weak in the brains of dogs with mild age-related changes. On other hand, both MT-III immunoreactivity and signals for MT-III mRNA were demonstrated in neurons in the brain regardless of the intensity of the age-related changes.

Beagle犬表现出多种与年龄相关的脑形态学改变，包括神经元营养不良性轴突改变、星形胶质细胞增生和以大脑皮质中s斑块形成为特征的淀粉样变性。为研究金属硫蛋白（MT）在脑中的作用，研究了老年Beagle犬脑中MT的表达模式。MT-I和-II之间的免疫反应以及MT-I和-II mRNA的信号显示在肥大的星形胶质细胞在大脑中与严重的年龄相关的变化。这些MT-I和-II阳性的星形胶质细胞主要分布在丘脑、大脑皮质和髓质，在那里观察到与年龄相关的变化，包括铁和脂褐素沉积。相比之下，MT-I II的表达是相当弱的狗的大脑与轻微的年龄相关的变化。另一方面，MT-III免疫反应性和MT-III mRNA的信号在脑神经元中被证明，无论年龄相关变化的强度如何。

项目成果

Kojima,S.: "Localization of Metallothioneins-Iz-II and-III in the Brain of Aged Dog" Journal of Veterinary Medical Science. (1999)

Kojima,S.：“金属硫蛋白-Iz-II 和-III 在老年狗大脑中的定位”《兽医医学科学杂志》。

Kojima, S.: "Localization of Metallothioneins-I &-II and -III in the Brain of Aged Dog"Journal of Veterinary Medical Science. 61. 343-349 (1999)

小岛，S.：“金属硫蛋白-I 的定位

Kojima,S., Shimada,A., Morita,T., Yamano,Y. and Umemura,T.: "Localization of Metallothioneins-I & -II and -III in the Brain of Aged Dog"Journal of Veterinary Medical Science. 61. 343-349 (1999)

小岛，S.，岛田，A.，森田，T.，山野，Y。