Identification and functional analysis of a novel receptor for an odorant, benzaldehyde, in Caenorhabditis elegans.

秀丽隐杆线虫能探测到许多化学物质，这些化学物质可以是引诱剂或驱避剂。尽管已经报道了不同的七跨膜受体，但它们是C.在线虫中，只有Odr-10被鉴定为引诱剂双乙酰的受体。C06H5.7编码七跨膜结构域受体以及odr-10。然而，它还没有被预测为一个候选的化学感受器。在本研究中，我们表明，C06H5.7可能编码的受体的气味苯甲醛。苯甲醛在低浓度下表现出吸引效应，在高浓度下表现出排斥效应。C06H5.7中的无效突变导致苯并咪唑特异性厌恶缺陷，但不是吸引缺陷。C06H5.7在ASH化学感觉神经元中表达，其感觉许多厌恶刺激。另一方面，C06H5.7既没有检测到AWA也没有AWC神经元，这是所需的特定子集的挥发性引诱剂的趋化性。C06H5.7 cDNA拯救了C06H5.7无效突变体的苯甲醛缺陷。有趣的是，在电生理分析中，C06H5.7对苯甲醛的类似物如3，4-二羟基苯甲醛、3，4-二羟基苯甲酸和4-羟基苯基乳酸有反应，但对苯甲醛本身无反应。结论是C. elegans通过ASH中的C06H5.7对苯甲醛排斥作出响应。此外，C.秀丽线虫可能检测到挥发性气味剂作为它们在粘膜中衍生的水溶性类似物。

The nematode Caenorhabditis elegans.detects many chemicals, wchich can be attractants or repellents. Although divergent seven transmembrane receptors have been reported candidates chemosensory receptors in C. elegans, only Odr-10 was identified as the receptor for an attractant, diacetyl. C06H5.7 encodes a seven transmembrane domain receptor as well as odr-10. However, it has not yet been predicted to be a candidate chemosensory receptor.In the present study, we show that C06H5.7 is likely to encode a receptor for the odorant benzaldehyde. Benzaldehyde exhibits attractive effects at low concentrations and repulsive effects at high concentrations. A null mutation in C06H5.7 caused a benzaldehyde-specific aversion defect, but not an attraction defect. C06H5.7 was expressed in the ASH chemosensory neurons, which senses many aversive stimuli. On the other hand, C06H5.7 was detected in neither AWA nor AWC neurons, which are required for chemotaxis to specific subsets of volatile attractants. The C06H5.7 cDNA rescued the benzaldehyde defect of C06H5.7 null mutant. Interestingly, C06H5.7 responded to analogues of bezaldehyde such as 3, 4-dihydroxybenzaldehyde, 3, 4-dihydroxybenzoic acid and 4-hydroxyphenyl lactic acid, but not bezaldehyde itself in electrophysiological analysis. In conclusion, C. elegans responded to benzaldehyde repulsion via C06H5.7 in ASH. Furthermore, C. elegans might detect the volatile odorant as their water-soluble analogs derived in mucosa membrane.