Identification of membrane targets for 15-deoxy Δ^<12,14>-prostaglandin J_2

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We found that specific binding sites of 15-deoxy-Δ^<12,14>-prostaglandin J_2 (15d-PGJ_2) was involved in the neurodegeneration. A proteomic approach was used to identify protein targets for 15d-PGJ_2 in the plasma membrane. By using biotinylated 15d-PGJ_2, eleven proteins were identified as biotin-positive spots and classified into three different functional proteins : glycolytic enzymes (Enolase2, pyruvate kinase M1 (PKM1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)), molecular chaperones (heat shock protein 8 and T-complex protein 1 subunit α), cytoskeletal proteins (Actin β, F-actin-capping protein, Tubulin β and Internexin α). GAPDH, PKM1 and Tubulin β are Aβ-interacting proteins.