Construction of Tissue Engineered Vascular Autograft and Heart Valve utilizing Biodegradable Scaffold

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This study demonstrates the evaluation of tissue-engineered vascular autografts (TEVAs) and Tissue Engineered Heart Valves (TEHV) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using an experimental animal model.MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. On the other hanc, TEHV were constructed with mixed venous cells which were cultured in vitro followed by seeding onto the biodegradable scaffold which was constructed with polyglycolic acid. TEVAs and TEHV were analyzed biochemically, biomechanically and histologically after implantation.TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification. And TEHV tolerated RV pressure after implantation.These results indicate that TEVAs and TEHV are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.