Developing the soil improvement material for the replanting failure by excrement sludge with Bacillus subtilis, clear water sludge and mushroom abolishment fungus floor

为了防止日本所有农田的补种失败，日本使用了各种杀虫剂和消毒剂。然而，这些农药对环境和农民的健康都是不利的。本研究试图利用芽孢杆菌的抗菌活性和抗菌活性来预防再植失败。以快滤厂清水污泥和蘑菇工业废菌地坪为原料，首先将这3种原料混合，在实验室中温条件下继续好氧发酵。在发酵开始时，霉菌和各种细菌菌落生长，但经过2-3周的培养，霉菌和各种细菌菌落消失，芽孢杆菌。占主导地位。接下来，对土壤改良剂进行了试制，证实为芽孢杆菌属。使成优势菌落生长到1.0E+08个菌落/g。超过1.0E+07菌落/g，可克服补植失败。2006年春季，研制了土壤改良剂，并在山药耕地上进行了补植失败的抑制试验。结果表明，新产生的芽孢杆菌具有较好的发酵效果。优势土壤改良剂在实际耕作条件下能有效抑制补植失败。

Various pesticides and disinfectants are used in order to prevent the replanting failure on every farmland in Japan. However, these agricultural chemical are not good for environment and farmer's health. In this research, it was tried to prevent the replanting failure by using antimicrobial and antibiotic activity of the bacillus sp..Dry sludge of the human excreta treatment plant where the bacillus sp. was dominant, clear water sludge of rapid filtration plant and mushroom industry abolishment fungus floor were used as a raw material of new soil conditioner.At first, these 3 kinds of raw material were mixed, and the aerobic fermentation continued under medium-temperature in the laboratory. At the fermentation start, mold and various bacteria colonies grew, but after 2 - 3 weeks cultivation, mold and various bacteria colonies disappeared and the bacillus sp. dominated. Next, the trial manufacturing of the soil conditioner, it was confirmed that the bacillus sp. made into the dominance grew up to 1.0E+08 colonies /g. It is said if the number of Bacillus sp. exceed 1.0E+07 colonies/g, replanting failure could be overcomed.Then the soil conditioner was produced experimentally in the spring 2006, and inhibition test of the replanting failure was carried out on a Chinese yam plowed field. The result was good, and from this result, it was proved that the newly produced bacillus sp. dominant soil conditioner was effective for the replanting failure inhibition in actual plowed field.