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Comparison of competition types among experimental populations of Callosobruchus maculates using the true phylogenetic tree

使用真实系统发育树比较 Callosbruchus maculates 实验种群之间的竞争类型

基本信息

批准号：
17570014
负责人：
TOKUNAGA Yukihiko
金额：
$ 1.92万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

I first applied several DNA micro-satellite markers for Acanthoselides and Zabrotes species to Callosobruchus maculatus strains, but none of them worked properly. Next, I applied probes derived from cDNA pattern of soy digesting enzymes discovered in a C.maculatus strain against my laboratory strains, but none of them worked properly. Finally I applied several RAPD markers developed for an Indian strain of C.maculatus against to my laboratory strains. Variation in these RAPD markers was reported to correspond to variation in body color patterns of the C.maculatus strains. However, there was no clear correspondence between variation in RAPD patterns and the body color variation among the laboratory strains. Instead, the variation in RAPD patterns was clearly explained by the difference in geographic origin of each strain. The RAPD pattern variation was also explained by heterogeneity in competition types among the laboratory strains.Crossing experiments between the most-extreme contest … More and the most-extreme scramble strains of C.maculatus showed that competition type was additively genetically determined. Contrarily, genetic mechanism of developmental period was nonlinear and even showed heterosis. As the results, variation in competition type among laboratory strains was not simply determined by difference in body size, or developmental heterogeneity. Black body color of a black mutant strain was disappeared with injection of JH to larval bodies.C.maculatus larvae retain interference ability inside beans. However, most of the geographic strains in my laboratory showed scramble competition, and hence there should be some mechanisms to moderate interference behavior among larvae interacting inside a bean. Heterogeneity in larval interference behavior was clearly explained by variation in wall-making behavior inside beans in both comparison among geographic strains and comparison among lines derived from a geographic strain. The wall-making behavior was also additively genetically determined, which well corresponded to the genetic pattern of competition types. Less

我首先应用了几个DNA微卫星标记Acanthoselides和Zabrotes物种的Callosobruchus maculatus菌株，但没有一个工作正常。接下来，我将从在斑隐翅虫菌株中发现的大豆消化酶的cDNA模式中衍生的探针应用于我的实验室菌株，但它们都不能正常工作。最后，我应用了几个RAPD标记开发的印度菌株的C.maculatus对我的实验室菌株。据报道，这些RAPD标记的变化对应于C.maculatus菌株的体色模式的变化。然而，没有明确的对应关系之间的变化，在RAPD模式和体色的变化在实验室菌株。相反，在RAPD模式的变化清楚地解释了每个菌株的地理来源的差异。实验室菌株间竞争类型的异质性也解释了RAPD图谱的变异。 ...更多信息而最极端的竞争品系表明，竞争类型是由加性遗传决定的。生育期的遗传机制是非线性的，甚至表现出杂种优势。结果表明，实验室品系之间竞争类型的变化不仅仅是由个体大小或发育异质性的差异决定的。将JH注射到幼虫体中，黑色突变体的黑色体色消失，斑点拟谷盗幼虫保留了在豆内的干扰能力。然而，在我的实验室中的大多数地理菌株表现出争夺竞争，因此应该有一些机制来调节幼虫之间的干扰行为在豆内相互作用。幼虫干扰行为的异质性清楚地解释了在地理株系之间的比较和来自一个地理株系之间的比较中，在豆类内部的造壁行为的变化。造壁行为也是加性遗传决定的，与竞争类型的遗传模式相一致。少