Studies on the genetic effect in termite caste determination

白蚁种姓判定的遗传效应研究

基本信息

批准号：
17570013
负责人：
KITADE Osamu
金额：
$ 2.3万
依托单位：
Ibaraki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570013/
关键词：
termite caste determination mechanism gene parthenogenesis

项目摘要

1. Parthenogenetic ability of termites and caste ratio of the parthenogens:Six species of termites (four Reticulitermes spp., Z. nevadensis, N. koshunensis) and regional populations of R. speratus were investigated on their parthenogenetic ability. The former sex species could not reproduce by parthenogenesis, but individuals from every local population of R. speratus could. All the parthenogens developed into female nymphs. The phylogeny of the local populations of R. speratus was inferred by molecular phylogenetic analysis of mitochondrial genes.2. Test of genetic caste determination model:Using R. kanmonensis and R. okinawanus, the genetic caste getermination model was tested. We carried out four types of crossing experiments using male and female nymphoid and ergatoid neotenic reproductives. Eggs were reared by male workers. In the former species the realized caste/sex ratios of the offspring did not differ from the expected values of the genetic model. The almost the same results were obtained in R. okinawanus, suggesting that a common mechanism regulate the caste ratio in the genus Reticulitermes.3. Search for the caste-and sex-lined genetic markers:We carried out AFLP analysis to find caste or sex specific genetic markers. Using 21 selective primer pairs, we found 316 polymorphic AFLP loci, but could not find caste specific bands. However, one male specific AFLP locus was found, which probably on the Y-chromosome. We also set up the experimental conditions of pulsed-field gel electrophoresis to separate sex chromosomes.4. Genetic structure and caste ratio in the field colony:We investigated of the genetic structures of R. speratus in Tokai, Ibaraki pref. using microsatellite and mt-DSCP markers. We estimated colony boundaries and examined caste compositions of the field colonies.

1。白蚁的单位生殖能力和对单毒素的种姓比：六种白蚁（四种网状属属，Z。Nevadensis，N。Koshunensis）和speratus的区域种群的骨py虫生殖能力进行了研究。以前的性别物种无法通过孤thenenogenogeny繁殖，但是来自R. speratus的每个当地人口都可以。所有的骨蛋白成分发展为雌性若虫。通过线粒体基因的分子系统发育分析，推断出斑点局的局部种群的系统发育。2。遗传种姓确定模型的测试：使用R. kanmonensis和R. okinawanus，测试了遗传种姓getermination模型。我们使用雄性和雌性男性和肾上腺样生殖进行了四种类型的交叉实验。鸡蛋由男工人饲养。在前一种物种中，后代实现的种姓/性别比与遗传模型的预期值没有差异。在冲绳菌中获得了几乎相同的结果，这表明一种共同的机制调节了网状属中的种姓比3。搜索种姓和性别衬里的遗传标记：我们进行了AFLP分析以找到种姓或性别特定的遗传标记。使用21个选择性底漆对，我们发现了316个多态性AFLP基因座，但找不到种姓特定的带。但是，发现一个男性特异性AFLP基因座，可能在Y染色体上。我们还设置了脉冲场凝胶电泳的实验条件，以分离性别染色体4。田间菌落中的遗传结构和种姓比：我们调查了伊巴拉基预先托卡的史佩拉图斯的遗传结构。使用微卫星和MT-DSCP标记。我们估计了殖民地边界，并检查了田间菌落的种姓组成。