Development of new breeding methods in chickens without use of the antibacterial medicine based on control of gut immune function

基于肠道免疫功能控制的不使用抗菌药物鸡育种新方法的开发

• 批准号: 17580232
• 负责人: TAKAHASHI Kazuaki
• 金额: $ 2.3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580232/
• 关键词: chickens; gut immunity; antibiotics; cytokine

1. Developmental changes in maturation of immune related cells in the gut during the first 2 weeks post-hatch were determined in broiler chickens fed a corn-soybean meal based (CS) diet, semi-purified diet with high fat (HF) or high carbohydrate (HC). Expression of CD3 and IFN-y mRNA increased during 3 to 7 days of age in three sections of the gut determined. Further increase in CD3 was observed in mid and hind gut during 7 to 14 days. When chicks were fed the CS diet, expression of IL-2 mRNA in fore gut did not increased with age during the experimental periods, but the expression in mid section increased at 14 days of age and in the hind section at 7 days of age. Expression of iNOS mRNA was lowered at 3 days of age in the fore and mid gut but the expression was constant at the other days of age. The changes in iNOS expression in the hind gut were similar as that of CD3 mRNA. TLR-2 mRNA expression increased during 3 to 7 days of age in three section of the gut, while TLR-4 expression increased during 0 to 3 days of age. Chicks fed the HC diet showed higher IL-2 but lower IFN-y mRNA expression than birds fed the CS diet and had age related increase in IL-2 expression in the fore gut. Feeding the HF diet tended to decrease the expressions determined except TLR-2, regardless of age and section of the gut determined.2. The complete open reading frames of chicken TL1A contained 717 base pairs with an open leading frame, predicting a protein of 239 amino acids. ChTL1A mRNA expression changing by LPS stimulation was observed in most tissues, in particularly the highest levels were found in spleen and adrenal. Furthermore, recombinant ChTL1A has a dose-dependent cytotoxic activity to both L929 and chicken fibroblast.

1.本研究测定了以玉米-豆粕（CS）为基础的日粮、高脂（HF）半纯化日粮和高碳水化合物（HC）半纯化日粮饲养的肉仔鸡在孵化后2周内肠道免疫相关细胞成熟的发育变化。3和IFN-γ mRNA的表达增加，在3至7日龄在三个部分的肠道测定。在7至14天期间，在中肠和后肠中观察到CD 3的进一步增加。饲喂CS日粮时，IL-2 mRNA在前肠中的表达量在试验期间不随日龄增加而增加，但在14日龄中段和7日龄后段的表达量分别增加。3日龄前肠和中肠中iNOSmRNA的表达降低，但在其他日龄的表达是恒定的。后肠iNOS表达的变化与CD 3 mRNA的变化相似。TLR-2 mRNA在3 ~ 7日龄肠段中的表达均呈上升趋势，TLR-4 mRNA在0 ~ 3日龄肠段中的表达呈上升趋势。与饲喂CS饮食的鸡相比，饲喂HC饮食的鸡显示出较高的IL-2但较低的IFN-γ mRNA表达，并且在前肠中具有与年龄相关的IL-2表达增加。饲喂HF饲料有降低TLR-2表达的趋势，但TLR-2的表达量与年龄和肠段无关.鸡TL 1A蛋白的完整开放阅读框架包含717个碱基对，并有一个开放的前导框架，预测蛋白质由239个氨基酸组成。LPS刺激后，ChTL 1AmRNA在大部分组织中表达均发生变化，其中以脾和肾上腺的表达量最高。此外，重组ChTL 1A对L929和鸡成纤维细胞都具有剂量依赖性的细胞毒活性。

项目成果

Developmental changes in mRNA expression in immune-associated cells of intestinal tract of broiler chickens after hatch and by dietary modulation.

肉鸡孵化后和饮食调节肠道免疫相关细胞 mRNA 表达的发育变化。

• 发表时间: 2007
• 期刊: Animal Science Journal 78・5(in press)
• 作者: M.Tohno;T.Shimazu;W.Ueda;D.Anzawa;H.Aso;J.Nishimura;Y.Kawai;et al.;K.Takahashi;K.Matsushita;K.Takahashi
• 通讯作者: K.Takahashi

Developmental changes in mRNA expression in immune-associated cells of intestinal tract of broiler chickens after hatch and by dietary modulation

孵化后和日粮调节下肉鸡肠道免疫相关细胞 mRNA 表达的发育变化

• 发表时间: 2007
• 期刊: Animal Science Journal 78・5(In press)
• 作者: M.Tohno;T.Shimazu;W.Ueda;D.Anzawa;H.Aso;J.Nishimura;Y.Kawai;et al.;K.Takahashi
• 通讯作者: K.Takahashi

Molecular cloning and functional characterizations of chicken TL1A.

鸡TL1A的分子克隆和功能表征。

• 发表时间: 2005
• 期刊: Developmental and Comparative Immunology 29
• 作者: M.Tohno;T.Shimazu;W.Ueda;D.Anzawa;H.Aso;J.Nishimura;Y.Kawai;et al.;K.Takahashi;K.Matsushita;K.Takahashi;K.Matsushita;K.Takahashi;K.Takahashi;K.Takahashi;T.Takimoto
• 通讯作者: T.Takimoto

Effects of adequate or marginal excess of dietary methionine hydroxy analogue free acid on growth performance, edible meat yield and inflammatory response in female broiler chickens.

日粮中适量或略微过量的蛋氨酸羟基类似物游离酸对雌性肉鸡生长性能、可食肉产量和炎症反应的影响。

• 发表时间: 2007
• 期刊: Journal of Poultry Science. 44・3(in press)
• 作者: M.Tohno;T.Shimazu;W.Ueda;D.Anzawa;H.Aso;J.Nishimura;Y.Kawai;et al.;K.Takahashi;K.Matsushita;K.Takahashi;K.Matsushita
• 通讯作者: K.Matsushita

Nutritional control of inflammatory responses in broiler chicken.

肉鸡炎症反应的营养控制。

• 发表时间: 2006
• 期刊: Journal of Integrated Field Science 3
• 作者: M.Tohno;T.Shimazu;W.Ueda;D.Anzawa;H.Aso;J.Nishimura;Y.Kawai;et al.;K.Takahashi;K.Matsushita;K.Takahashi;K.Matsushita;K.Takahashi;K.Takahashi
• 通讯作者: K.Takahashi