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Regulation of immune system in newly hatched chicks by control of cytokine production

通过控制细胞因子的产生来调节新孵化雏鸡的免疫系统

基本信息

批准号：
13660279
负责人：
TAKAHASHI Kazuaki
金额：
$ 2.24万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660279/
关键词：
Cytokine production and gene expression Immune regulation Nutritional manipulation Newly hatched chick 孵化後の栄養

项目摘要

This research was conducted to enhance immunity especially splenic T cells function through the regulation of cytokine production during first weeks afler hatch of chicks by nutritional manipulation. Proliferative response and interleukin 2 production estimated by mRNA expression in splenic lymphocytes was low during first weeks after the hatch, but interferon-gamma was not. Nutrients deficiency markedly lowered the T cell functions in not only during first week after the hatch but also in the later of growth stage. Residual egg yolk may contribute to maintain the functions. DHA in egg yolk was an essential nutrient to enhance T cell function, but it was not enough to completely enhance the function. The other fat soluble substance(s) would be required. Conjugated linoleic acid and xylitol are novel nutrients to enhance immunity, and a single oral administration of xylitol with newly hatched chicks improved latter growth and T cell functions. Flow cytometry analysis showed suggested that dietary DHA and conjugated linoleic acid may be affect T cell differentiation in thymus. Highly homologous chicks' interleukin 4 to mammals, produced by type 2-T cell has not been identified at the present research. The method for determination of inducible NOS, Cox-2 and PPAR-gamma mRNA by real time PCR has been developed. The result suggests that an enhancement of immunity especially splenic T cells function through the regulation of cytokine production during first weeks after hatch of chicks by nutritional manipulation is available to due change in cytokine production by nutrients such as DHA, conjugated linoleic acid and xylitol, and the method developed in this research is useful to understand the T cell functions in newly hatched chicks.

本研究旨在通过营养调控来调节雏鸡出壳后1周内细胞因子的产生，从而提高雏鸡免疫功能，尤其是脾T细胞的功能。脾淋巴细胞中的mRNA表达估计的免疫反应和白细胞介素2的生产是低的，在孵化后的第一周，但干扰素-γ不是。营养素缺乏不仅在孵化后第一周，而且在生长后期都显著降低了T细胞功能。残留的蛋黄可能有助于维持功能。蛋黄中的DHA是增强T细胞功能的必需营养素，但不足以完全增强功能。需要其他脂溶性物质。共轭亚油酸和木糖醇是增强免疫力的新型营养素，并且对刚孵化的雏鸡单次口服木糖醇改善了后期生长和T细胞功能。流式细胞仪分析表明，DHA和共轭亚油酸可能影响胸腺T细胞分化。鸡IL-4与哺乳动物高度同源，由2型T细胞产生，目前尚未发现。建立了真实的时间PCR检测诱导型NOS、考克斯-2和PPAR-gamma mRNA的方法。结果表明，营养调控可通过调节雏鸡出壳后1周内细胞因子的产生来增强免疫功能，尤其是脾T细胞的功能，这可能是由于DHA、共轭亚油酸和木糖醇等营养物质改变了雏鸡细胞因子的产生，本研究建立的方法有助于了解雏鸡出壳后T细胞的功能。