Investigation of the mechanism of Maize flowering and its signal transduction

玉米开花机制及其信号转导研究

基本信息

批准号：
17580297
负责人：
KOZAKI Akiko
金额：
$ 2.24万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580297/
关键词：
plant development flowering Maize transcriptional regulation 分化・発生

项目摘要

The maize INDETERMINATE 1(ID1) gene is a key regulator of the transition to flowering and founding member of a transcription factor gene family that encodes a protein with distinct arrangemeni of zinc finger motif. To investigate the mechanism how ID1 gene regulates flowering, we have analyzed1) the function of ID family genes in Arabidopsis and rice2) the transcriptional activity of ID1 protein3) the target candidate genes of ID11) We have made several transgenic plants overexpressing ID family genes or plants in which expression of ID family genes were reduced by RNAi, and analyzed the phenotype of these transgenic plants. The results indicated that one of ID family gene in rice, OsID is possibly the ortholog of ID1 and there seems to be no ortholog of ID1 in Arabidopsis.2) We analyzed the transcriptional activity of ID1 protein using yeast one-hybrid or two-hybrid system and found that full length of ID1 protein is a repressor, however, other ID family proteins are activator. The results indicated that ID1 is functionally different from other ID family gene.3) Because we found that OsID is the ortholog of ID1 gene, we analyzed the expression of flowering genes in transgenic rice which is overexpressing OsID or RNAi plants with reduced level of OsID and found that expression of some genes are affected by expression of OsID.

玉米不确定的1（ID1）基因是转录因子基因家族的开花和创始成员的关键调节因子，该基因家族编码具有锌指基序的蛋白质的蛋白质。 To investigate the mechanism how ID1 gene regulates flowering, we have analyzed1) the function of ID family genes in Arabidopsis and rice2) the transcriptional activity of ID1 protein3) the target candidate genes of ID11) We have made several transgenic plants overexpressing ID family genes or plants in which expression of ID family genes were reduced by RNAi, and analyzed the phenotype of these transgenic plants. The results indicated that one of ID family gene in rice, OsID is possibly the ortholog of ID1 and there seems to be no ortholog of ID1 in Arabidopsis.2) We analyzed the transcriptional activity of ID1 protein using yeast one-hybrid or two-hybrid system and found that full length of ID1 protein is a repressor, however, other ID family proteins are activator.结果表明，ID1在功能上与其他ID家族基因的功能不同。3），因为我们发现oSID是ID1基因的直系同源物，我们分析了转基因水稻中开花基因的表达，该水稻过表达oSID或RNAi植物的渗透水平降低，并发现某些基因的表达受到肌肉表达的影响。