Micro-and macro-applications of moss protonema culture to environmental protection.

苔藓原丝培养在环境保护中的微观和宏观应用。

• 批准号: 17580287
• 负责人: TAKAHARA Yoshinori
• 金额: $ 2.11万
• 依托单位: Nagaoka University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17580287/
• 关键词: Environmental polution; Plant; Bioassay; Urban environment; Green building; Moss protonema; Plant tissue culture; Reduction of CO2 emisson; コケ; 原糸体; 建築物緑化

Moss protonema is the first phase tissue in its life cycle. A cell emerged from a pollen grows into a protonema of serial cell chain structure by two type cell divisions. One is tip cell division that causes chain elongation and the other is branching division that makes new tip cells. Moss protonema has suitable feature for bioassay to test environmental polutions as protonema shows very fast growth and all cells of protonema are exposed to surrounding environment. At the same time, its fast growth is favorable for building greening. In this study, investigations of these two applications of moss protonemas, bioassay and green buildings, were performed, that is, how to use moss protonemas in the applications. Effect of two heavy metal ions, as serious polutant in Japan, on moss protonema growth was measured. Protonemas of five moss species were used for the experiment, and high sensitivities of moss protonemas against heavy metal ions and interspecies difference were obserbed. Methodology of moss preparations for building greening was researched. Proliferation of moss protonema by large-scale liquid shaking culture was successful by using magna flex type spinner flasks, and propageted protonemas could be clung to some sheets by lotator-culture with base-sheets. It was indicated that moss protonemas have an aptitude for both bioassay and building greening materials.

苔藓原丝体是其生命周期的第一阶段组织。从花粉中出现的细胞通过两种类型的细胞分裂生长成具有连续细胞链结构的原丝体。一种是导致链伸长的尖端细胞分裂，另一种是产生新尖端细胞的分支分裂。苔藓原丝体具有适合生物测定以测试环境污染的特征，因为原丝体生长非常快并且所有原丝体细胞都暴露于周围环境。同时，其快速增长有利于建筑绿化。本研究针对苔藓原丝体生物测定和绿色建筑这两个应用进行了研究，即如何在应用中使用苔藓原丝体。测量了日本严重污染物两种重金属离子对苔藓原丝体生长的影响。采用5种苔藓植物的原丝体进行实验，观察到苔藓原丝体对重金属离子的高敏感性和种间差异。对建筑绿化苔藓制剂的方法进行了研究。使用magna flex型旋转烧瓶，通过大规模液体振荡培养成功地增殖了苔藓原丝体，并且通过带有基片的分选器培养，繁殖的原丝体可以粘附在一些片材上。表明苔藓原丝体具有生物测定和建筑绿化材料的适应性。

项目成果

Studies on bioassey system by measurement of moss protnema growth. (in Japanese)

通过测量苔藓原丝生长的生物测定系统的研究。

• 发表时间: 2006
• 期刊: Proceeding of the 35^<th> Annual Meeting of the Bryological Society of Japan
• 作者: Amirta;r.;Tanabe;T.;Watanabe;T.;Honda;Y.;Kuwahara;M.;Watanabe;T.;Daisuke Hoshi;Aiko Oshio;大塩愛子;星大祐;Daisuke Hoshi;Aiko Oshio;Aiko Oshio;Daisuke Hoshi;Aiko Oshio;Daisuke Hoshi
• 通讯作者: Daisuke Hoshi

Development of bioassay system by measurement of moss protonema growth.

通过测量苔藓原丝生长来开发生物测定系统。

• 发表时间: 2006
• 期刊: Proceedings of the 6^<th> 21^<st> Century COE International Symposium on Global Renaissance by Green Energy Revolution
• 作者: Amirta;r.;Tanabe;T.;Watanabe;T.;Honda;Y.;Kuwahara;M.;Watanabe;T.;Daisuke Hoshi
• 通讯作者: Daisuke Hoshi

Study of building greening by large scale culture of moss protonema.

大规模培养苔藓原丝体对建筑绿化的研究

• 发表时间: 2006
• 期刊: Proceedings of the 6^<th> 21^<st> Century COE International Symposium on Global Renaissance by Green Energy Revolution
• 作者: Amirta;r.;Tanabe;T.;Watanabe;T.;Honda;Y.;Kuwahara;M.;Watanabe;T.;Daisuke Hoshi;Aiko Oshio
• 通讯作者: Aiko Oshio

Fundamental study on application of moss protonema cultures for building greening.

苔藓原丝体培养物在建筑绿化中应用的基础研究

• 发表时间: 2006
• 期刊: Proceedings of the 7^<th> 21^<st> Century COE International Symposium on Global Renaissance by Green Energy Revolution
• 作者: Amirta;r.;Tanabe;T.;Watanabe;T.;Honda;Y.;Kuwahara;M.;Watanabe;T.;Daisuke Hoshi;Aiko Oshio;大塩愛子;星大祐;Daisuke Hoshi;Aiko Oshio
• 通讯作者: Aiko Oshio

コケ原糸体の大量培養による建物緑化の研究

苔藓原丝体大规模培养建筑绿化研究

• 发表时间: 2006
• 期刊: 日本蘚苔類学会第35回大会講演要旨集
• 作者: Amirta;r.;Tanabe;T.;Watanabe;T.;Honda;Y.;Kuwahara;M.;Watanabe;T.;Daisuke Hoshi;Aiko Oshio;大塩愛子
• 通讯作者: 大塩愛子