補助人工心臓下の虚血性心筋症に対する平滑筋細胞移植の効果

本研究使用同系Lwis大鼠。首先，使用6-0 Prolene结扎左前降支动脉，以获得雌性大鼠的前壁心肌梗死。在此程序之后，重复超声心动图检查心脏功能。心肌梗死后4周，进行超声心动图检查，如果25%的心肌梗死，则大鼠进入下一步。全麻下摘取梗死心脏作为供体心脏，植入受体腹部，用9-0 Prolene将供体升主动脉和肺动脉与腹主动脉和下腔静脉吻合。T组同时移植平滑肌细胞。移植后4周，取心脏，用Langendorff装置测定心功能，计数移植的雄核细胞，并进行组织病理学检查，Langendorff研究中，T组心脏功能明显优于C组（对照组）。此外，移植到梗死心肌中的雄性平滑肌细胞的百分比约为15%。T组心功能优于C组的原因之一是T组的小动脉数目多于C组，对心功能有较好的影响。这一概念可能适用于临床环境，例如患有心力衰竭的患者是否可以在平滑肌细胞移植后放弃LVAD植入。

Syngeneic Lwis rats were used for this study. First, left anterior descending artery was ligated using 6-0 Prolene to obtain anterior myocardial infarction in the female rats. After this procedure, echocardiography was repeated to examine cardiac function. 4 weeks after myocardial infarction, echocardiography was done, and if 25%of myocardium was infarcted, that rat went to next step. Under general anesthesia, infarcted hearts were extirpated as donor heart, which was then implanted into recipient abdomen, where ascending aorta and pulmonary artery of donor were anastomosed to abdominal aorta and inferior vena cava using 9-0 Prolene. Simultaneously,, smooth muscle cells were transplanted in group T. 4weeks after heterotopic transplantation, hearts were excised in order to measure cardiac function with langendorff apparatus, to count transplanted male cells, and to do histopathological examination.In Langendorff study, hearts in group T showed better cardiac function than those in group C (control) significantly. In addition, the percentage of male smooth muscle cells transplanted into infarcted myocardium was about 15%. One of the reasons why cardiac function in group T was better than that in group C was that the number of artrioles in group T was higher than that in group C, so it has good impact on cardiac function.In conclusion, cell transplantation in infarcted myocardium could be effective in terms of cardiac function. This concept might be applied to clinical settings, such as whether or not the patients who suffered from heart failure, can wean from LVAD implantation after smooth muscle cell transplantation.