PROTECTIVE EFFECT OF HUMAN THIOREDOXIN AGAINST WARM ISCHEMIA REPERFUSION INJURY OF THE LUNG

人硫氧还蛋白对肺热缺血再灌注损伤的保护作用

• 批准号: 17591467
• 负责人: NAKAMURA Takayuki
• 金额: $ 2.24万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591467/
• 关键词: transplantation; surgery; stress; protein; translational research; トランスレーショナルリサーチ

Human thioredoxin (hTRX) is aredox-regulating protein with anti-oxidative effect by the dithiol/disulfide exchange reaction in the two cysteines in the active site. Warm ischemia reperfusion injury is a critical problem in lung transplantation. We hypothesized that inhalation or perfusion of recombinant hTRX would protect the lung s against warm ischemia. The effects of rhTRX inhalation and perfusion were investigated against pulmonary ischemia reperfusion injury. Inhalation of rhTRX by nebulizer showed no protective effects. Perfusion of high (350μg/m1) but not low (200μg/ml) dose of rhTRX ameliorated post-ischemia pulmonary hypertension. 50mM but not 10mM rhTRX reduced non-mercaptoalbumin to mercaptoalbumin These results suggest that perfusion of rhTRX might become a useful strategy for lung preservation in lung transplantation. More sophisticated inhalation methods may warrant further investigation.

人硫氧还蛋白（Human thioredoxin，hTRX）是一种氧化还原调节蛋白，其活性部位的两个半胱氨酸通过二巯基/二硫键交换反应而发挥抗氧化作用。热缺血再灌注损伤是肺移植中的一个关键问题。我们假设吸入或灌注重组hTRX可以保护肺免受热缺血。观察rhTRX吸入和灌流对肺缺血再灌注损伤的保护作用。雾化吸入rhTRX无保护作用。高剂量（350μg/ml）灌注rhTRX可改善缺血后肺动脉高压，而低剂量（200μg/ml）灌注rhTRX对肺动脉高压无明显影响。50 mM而不是10 mM rhTRX使非巯基白蛋白还原为巯基白蛋白。这些结果表明，rhTRX灌注可能成为肺移植中有用的肺保护策略。更复杂的吸入方法可能需要进一步研究。

项目成果

Isoflurane inhalation after circulatory arrest protects against warm ischemia reperfusion injury of the lungs

• DOI: 10.1097/01.tp.0000237207.73439.2e
• 发表时间: 2006-11-15
• 期刊: TRANSPLANTATION
• 影响因子: 6.2
• 作者: Fujinaga, Takuji;Nakamura, Takayuki;Bando, Toru
• 通讯作者: Bando, Toru

Depletion of alveolar macrophages by clodronate-liposomes aggravates ischemia-reperfusion injury of the lung

• DOI: 10.1016/j.healun.2003.10.007
• 发表时间: 2005-01-01
• 期刊: JOURNAL OF HEART AND LUNG TRANSPLANTATION
• 影响因子: 8.9
• 作者: Nakamura, T;Abu-Dahab, R;Schäfers, HJ
• 通讯作者: Schäfers, HJ

Recombinant human thioredoxin suppresses lipopolysaccharide-induced bronchoalveolar neutrophil infiltration in rat

• DOI: 10.1016/j.lfs.2006.03.026
• 发表时间: 2006-08-15
• 期刊: LIFE SCIENCES
• 影响因子: 6.1
• 作者: Ueda, Shugo;Nakamura, Takayuki;Nakamura, Hajime
• 通讯作者: Nakamura, Hajime

Isoflurane inhalation after circulatory arrest protects warm ischemia reperfusion injury of the lungs.

停循环后吸入异氟醚可保护肺部的热缺血再灌注损伤。

• 发表时间: 2006
• 期刊: Transplantation 82(9)
• 作者: Fujinaga T;et al.
• 通讯作者: et al.

Protecitve effect of a nebulized beta2-adrenoreceptor agonist in warm ischemic-reperfused rat lungs.

雾化β2-肾上腺素受体激动剂对热缺血再灌注大鼠肺部的保护作用。

• 发表时间: 2006
• 期刊: Ann. Thorac. Surg. 82(2)
• 作者: Chen F;et al.
• 通讯作者: et al.