Pathology and treatment for osteoarthritis-efficient use of endogenous insulin-like growth factor-1

骨关节炎的病理学和治疗——有效利用内源性胰岛素样生长因子-1

• 批准号: 17591582
• 负责人: MATSUMOTO Tomoko
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591582/
• 关键词: Osteoarthritis; Insulin-like growth factor; articular cartilage; cartilage repair; IGF-結合蛋白

The aim of this study was to clarify the effects of insulin-like growth factor (IGF)-I on cartilage metabolism, and finally to investigate the treatment for osteoarthritis. IGF-I was added to the culture medium of human articular chondrocytes with/without interleukin (IL)-1 or dexamethasone. Total RNA was extracted from the cell layer, and mRNA expression for aggrecan, type II collagen, matrix metalloprotease (MMP), IGF-I, IGF-binding proteins, and IGF-I receptor was examined using RT-PCR and real time-PCR. IGF-I induced the expression of aggrecan and type II collagen mRNA, and slightly decreased the inhibitory effect of IL-I or dexamethasone on mRNA expression for aggrecan. IGF-I showed little effects on MMP-3, -9, 13 expression. Further IL-1 induced the expression of IGF-I but decreased IGF-receptor. On the contrary, dexamethasone induced IGF-1 receptor but decreased IGF-I expression. mRNA expression for IGF-binding proteins was also regulated by both f IL-1 and dexamethasone These findings suggest that IGF-I promote synthesis of cartilage matrix, and protect articular cartilage from degradation. IGF-I might be one of the candidates for treatment of osteoarthritis.

本研究的目的是阐明胰岛素样生长因子（IGF）-I对软骨代谢的影响，并最终探讨骨关节炎的治疗。将IGF-I加入到有/无白细胞介素（IL）-1或地塞米松的人关节软骨细胞的培养基中。从细胞层提取总RNA，并使用RT-PCR和真实的时间PCR检测聚集蛋白聚糖、II型胶原、基质金属蛋白酶（MMP）、IGF-I、IGF-I结合蛋白和IGF-I受体的mRNA表达。IGF-I可诱导聚集蛋白聚糖和II型胶原mRNA的表达，并可轻度减弱IL-1或地塞米松对聚集蛋白聚糖mRNA表达的抑制作用。IGF-I对MMP-3、MMP-9、MMP-13表达无明显影响。IL-1可诱导IGF-1的表达，但降低IGF-1受体的表达。相反，地塞米松诱导IGF-1受体，但降低IGF-I表达。IL-1和地塞米松对IGF-1结合蛋白mRNA表达也有调节作用。提示IGF-1可促进软骨基质的合成，保护关节软骨免受降解。IGF-I可能是治疗骨关节炎的候选药物之一。

项目成果

Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis

• DOI: 10.1007/s00776-005-0943-3
• 发表时间: 2005-11-01
• 期刊: JOURNAL OF ORTHOPAEDIC SCIENCE
• 影响因子: 1.7
• 作者: Yabe, Y;Matsumoto, T;Shindo, H
• 通讯作者: Shindo, H

Cartilage Oligomeric Matrix protein

软骨寡聚基质蛋白

• 发表时间: 2006
• 期刊: Rheumatology 35
• 作者: Sakimura K;Matsumoto T;et al.;Matsumoto T
• 通讯作者: Matsumoto T

Analysis of polyethylene particles isolated from periprosthetic tissue of loosened hip arthroplasty and comaprison with radiographic appearance.

从松动的髋关节置换术的假体周围组织中分离出的聚乙烯颗粒的分析以及与放射学外观的比较。

• 发表时间: 2005
• 期刊: J Orthopaedic Science 10
• 作者: Koseki H;Matsumoto T. et al.
• 通讯作者: Matsumoto T. et al.

【関節炎マーカーの基礎と臨床】COMP (cartilage oligomeric matrix protein)

[基础和临床关节炎标志物] COMP（软骨寡聚基质蛋白）

• 发表时间: 2006
• 期刊: リウマチ科 35(6)
• 作者: Sakimura K;Matsumoto T;et al.;松本智子
• 通讯作者: 松本智子

Effects of insulin-like growth factor I on transforming growth factor betal induced chondrogenesis of synovium-derived mesenchymal stem cells cultured in a polyglycolic acid scaffold

胰岛素样生长因子I对转化生长因子β诱导的聚乙醇酸支架中培养的滑膜间充质干细胞软骨形成的影响

• 发表时间: 2006
• 期刊: Cell Tissues Organs 183
• 作者: Sakimura K;Matsumoto T;et al.
• 通讯作者: et al.