Signal transduction of TGF-b in rat articular chondrocytes

TGF-b在大鼠关节软骨细胞中的信号转导

• 批准号: 07671598
• 负责人: MATSUMOTO Tomoko
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671598/
• 关键词: chondrocytes; TGF-b; signal transduction; TGF β receptor; chondrocyte; two-hybrid cloning

Transforming growth factor (TGF) -b promotes the proliferation and differentiation of chondrocytes, and it is assumed to play a role in the repair of cartilage tissue. The mechanism of signal transduction of TGF-b is still unknown, although type I,II and III receptors of TGF-b have been cloned. TGF-b stimulates the synthesis of DNA in chondrocytes unlike the other cells, suggesting signal transduction of TGF-b in chondrocytes is different from the others. We found that TGF-b upregulated the induction of c-fos gene in chondrocytes, and that TGF-b response element of upstream of c-fos gene is -403 to -222 using CAT assay. We have made c-DNA library of rat articular chondrocytes and tried to clone the protein intermediating the signal transduction of TGF-b by two hybrid system. Several colonies were obtained and they are screening now, however, no positive data are obtained. Further studies are needed to find a novel protein.

转化生长因子(TGF) -b)促进软骨细胞的增殖和分化，并被认为在软骨组织的修复中发挥作用。TGF-b的信号转导机制尚不清楚，尽管已克隆出TGF-b的I、II和III型受体。TGF-b对软骨细胞DNA合成的刺激作用不同于其他细胞，提示TGF-b在软骨细胞中的信号转导不同于其他细胞。我们发现TGF-b上调了c-fos基因在软骨细胞中的诱导作用，CAT检测发现TGF-b在c-fos基因上游的应答元件为-403 ~ -222。我们建立了大鼠关节软骨细胞的c-DNA文库，并尝试用两种杂交系统克隆介导TGF-b信号转导的蛋白。获得了几个菌落，目前正在进行筛选，但未获得阳性数据。需要进一步的研究来发现一种新的蛋白质。

项目成果

T Matsumoto: "Identification and characterization of insulin-like growth factors (IGFs), IGF-binding proteins (IGFBPs) and IGFBP proteases in human synovial fluid" J Clin Endocr Metab. 81. 150-155 (1996)

T Matsumoto：“人类滑液中胰岛素样生长因子 (IGF)、IGF 结合蛋白 (IGFBP) 和 IGFBP 蛋白酶的鉴定和表征”J Clin Endocr Metab。

Usa T: "Correlation between suppression of c-myc and antiproliferative effect of transforming growth factor-b1 in thyroid carcinoma cell growth" Endocrinology. 135. 1378-1384 (1994)

Usa T：“甲状腺癌细胞生长中 c-myc 抑制与转化生长因子-b1 抗增殖作用之间的相关性”内分泌学。

Matsumoto T: "Identification and characterization of insulin-like growth factor(IGFs),IGFBPs and IGFBP proteases in human synovial fluid." Journal of Clinical Endocrinology and Metabolism. 81. 150-155 (1996)

Matsumoto T：“人类滑液中胰岛素样生长因子 (IGF)、IGFBP 和 IGFBP 蛋白酶的鉴定和表征。”

Matsumoto T: "Identification and characterization of insulin-like growth factors (IGFs),IGFBPs and IGFBP proteases in human synovial fluid." Journal of Clinical Endocrinology and Metabolism. 81. 150-155 (1996)

Matsumoto T：“人类滑液中胰岛素样生长因子 (IGF)、IGFBP 和 IGFBP 蛋白酶的鉴定和表征。”

T Matsumoto: "The mechanism of induction of insulin-like growth factor binding protein (IGFBP)-5 by IGFs in rat articular chondrocytes" Endocrine Journal. 43. s93-s95 (1996)

T Matsumoto：“大鼠关节软骨细胞中 IGF 诱导胰岛素样生长因子结合蛋白 (IGFBP)-5 的机制”内分泌杂志。