Prognostic factors in Ewing/PNET family tumors

我们用RT-PCR方法检测了8例Ewing/PNET家族肿瘤的神经营养受体（trkA、trkB、trkC）和神经营养因子（NGF、BDNF、NT-3）。因此，通过免疫组织化学分析这些因素，7/8例中trkA和trkB阳性，5/8例中NGF阳性。5例同时表达trkA和NGF，提示肿瘤生长的自分泌环机制。本研究中，这些因子在骨尤文肉瘤（ESB）和骨外尤文肉瘤（埃斯克）中的表达存在差异。我们应用寡核苷酸芯片技术分析了11例尤文肉瘤（ESB：5例，埃斯克：6例）中gdne基因表达谱的变化趋势。对54，676个基因表达谱进行聚类分析和树状图分析，结果表明基因表达谱与肿瘤部位无关。神经营养受体和神经营养因子基因的信号表达模式相对较低，但两者之间呈负相关。RT-PCR结果与寡核苷酸芯片分析的信号表达模式具有良好的相关性，但RT-PCR结果与免疫组化结果存在差异。进一步研究基因分析与更多的样本是强制性的。

We have identified neurotrophic receptors (trkA, trkB, trkC) and neurotrophic factors (NGF, BDNF, NT-3) in 8 cases of Ewing/PNET family tumors by RT-PCR. Consequently, these factors were analyzed by immunohistochemistry, trkA, and trkB were positive in 7/8 and, NGF was positive in 5/8. There were 5 cases co-expressing both trkA and NGF, which suggested autocrine loop mechanism in tumor growth. In this study, there were discrepancy in the expression of these factors between Ewing's sarcoma of bone (ESB) and extraskeletal Ewing's sarcoma (ESK). We performed oligonucleotide microarray analysis for 11 cases of Ewing sarcoma (ESB: 5 cases, ESK: 6 cases) to analyze the tendency of the gdne expression profile. The result of cluster analysis and tree view of 54, 676 genes expression profile had suggested that there was no relationship between gene expression profile and location of tumor. Signal expression pattern of genes of neurotrophic receptors and neurotrophic factors were relatively low, but inverse relationship were observed between them. We found up-regulated gene expressions in IGF1-R, ID2, CD99, and down-regulated in TGFB-R2.The results of RT-PCR and signal expression patterns by oligonucleotide microarray analysis were well-correlated, but there were discrepancies between the results of RT-PCR and immunohistochemistry. Further studies of gene analysis with more samples are mandatory.