Effects of local anesthetics on the soma and axon in cultured neuron of Lymnaea stagnalis

点击翻译按钮获取中文摘要

Purpose. To investigate the burst spikes (BS) induced by lidocaine in the ganglion of Lymnaea stagnalis; we used a multielectrode dish for extracellular recording in many points simultaneously.Methods. Ganglion of Lymnaea stagnalis was put in the multielectrode dish that has 64 planar microelectrodes. After the basal electrophysiological recordings of neuronal activity in the ganglion, the lidocaine concentrations were increased from 1 to 1000 μg・ml^<-1>. Results. A sequence of changes was observed. The initial stage was represented before first BS. Second stage was during first BS (lidocaine concentrations were between 18±3 and 105±69 μg・m1^<-1>). Third stage was the period before the second BS. The fourth stag: was during second BS (lidocaine concentrations were more than 670±254 μg・ml^<-1>). Basic unit frequency of firing exclude BS were 83±33%at first stage, 91±28% at second stage, 77±29 %at third stage, and 50±41 %at fourth stage (p<0.05) compared to control (100%). Conclusions. The results of this study showed that the lidocaine induced BS with low and high lidocaine concentrations and have different mechanisms in the central nervous system of Lymnaea stagnalis.