Identification of progression-related genes in prostate cancer and application to treatment

前列腺癌进展相关基因的鉴定及其在治疗中的应用

• 批准号: 17591669
• 负责人: MIZOKAMI Atsushi
• 金额: $ 2.24万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591669/
• 关键词: prostate cancer; androgen-dependent; VEGF; cDNA microarray; ホルモン非依存性

1.We established androgen-independent prostate cancer cell lines, LN-REC4 and LNCaP-SF from LNCaP cells.2.These cells grew very well when they were inoculated in castrated scid mouse subcutaneously. One of this reason was speculated that increased VEGF secretion.3.We identified genes that were regulated by DHT by cDNA microarray analysis. (screened 16,785 genes).4.We confirmed that these genes were really regulated by DHT by RT-PCR.5.There were 374 and 581 genes in LNCaP and up-regulated by DHT and 623 and 388 genes down-regulated genes. 105 genes were down-regulated genes in both cell lines.

1.建立了雄激素非依赖性前列腺癌细胞株LNCaP-4和LNCaP-SF。推测其原因之一可能是增加了VEGF的分泌。3.通过cDNA微阵列分析确定了受DHT调控的基因。5.在LNCaP中，分别有374和581个基因被DHT上调，623和388个基因被DHT下调。105个基因在两种细胞系中表达下调。

项目成果

Establishment and characterization of androgen-independent human prostate cancer cell lines, LN-REC4 and LNCaP-SF, from LNCaP

• DOI: 10.1111/j.1442-2042.2007.01532.x
• 发表时间: 2007-03-01
• 期刊: INTERNATIONAL JOURNAL OF UROLOGY
• 影响因子: 2.6
• 作者: Iwasa, Yoichi;Mizokami, Atsushi;Namiki, Mikio
• 通讯作者: Namiki, Mikio