Identification of genes associated with relapse of prostate cancer and gene therapy

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We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4).We characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer.We also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.