Identification of genes associated with relapse of prostate cancer and gene therapy

与前列腺癌复发相关的基因鉴定和基因治疗

• 批准号: 13671640
• 负责人: MIZOKAMI Atsushi
• 金额: $ 2.3万
• 依托单位: Kanazawa university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671640/
• 关键词: prostate cancer; androgen dependency; relapse; COX-2; 遺伝子治療

We inoculated LNCaP cells, androgen-sensitive human prostate cancer cells, onto 5-week aged scid mice subcutneously with 50 % matrigel. After tumor was increased visually, we castrated the mouse. First, tumor retarded for 2 weeks. Then it stated to enlarge. We sacrificed the mouse and took the tumor and subcultured. (LN-RECO). Next we inoculated the LN-RECO to the castrated mouse. Again we took the enlarged tumor and subcultured (LN-REC4).We characterized LN-REC4 cells. Although LN-REC4 cells were stimulated by androgen in vitro, the growth speed of those cells in the castrated mouse was faster than LNCaP cells, suggesting that LN-REC4 seemed to be a good model that reflect the relapse of prostate cancer.We also investigated the COX-2 gene since this gene seemed to be related with progression of prostate cancer. Overexpression of COX-2 gene in LNCaP cells induced cell proliferation in vitro and in vivo, and induced VEGF secretion.

我们将LNCaP细胞，雄激素敏感的人前列腺癌细胞，用50%基质胶皮下接种到5周龄scid小鼠上。待肿瘤明显增大后，将小鼠去势。首先，肿瘤延迟2周。然后它说要扩大。我们处死小鼠，取出肿瘤并进行传代培养。（LN-RECO）。接下来，我们将LN-RECO接种到去势小鼠。我们再次取出扩大的肿瘤并进行传代培养（LN-REC 4）。我们对LN-REC 4细胞进行了表征。虽然LN-REC 4细胞在体外被雄激素刺激，但其在去势小鼠体内的生长速度比LNCaP细胞快，这表明LN-REC 4细胞似乎是一个很好的反映前列腺癌复发的模型。考克斯-2基因在LNCaP细胞中的过表达在体外和体内均诱导细胞增殖，并诱导VEGF分泌。

项目成果

Fujita, H., Koshida, K., Keller, E.T., Takahashi, Y., Yoshimoto, T., Namiki, M., Mizokami, A: "Cyclooxygenase-2 promotes prostate cancer progression"The prostate. 53. 232-240 (2002)

Fujita, H.、Koshida, K.、Keller, E.T.、Takahashi, Y.、Yoshimoto, T.、Namiki, M.、Mizokami, A：“Cyclooxygenase-2 促进前列腺癌进展”前列腺。

Fujita, H., Koshida, K., Keller, E. T., Takahashi, Y., Yoshimito, T., Namiki, M., Mizokami, A: "Cyclooxygenase-2 promotes prostate cancer progression"The prostate. 53. 232-240 (2002)

Fujita, H.、Koshida, K.、Keller, E. T.、Takahashi, Y.、Yoshimito, T.、Namiki, M.、Mizokami, A：“Cyclooxygenase-2 促进前列腺癌进展”前列腺。

Cyclooxygenase-2 promotes prostate cancer progression

• DOI: 10.1002/pros.10152
• 发表时间: 2002-11-01
• 期刊: PROSTATE
• 影响因子: 2.8
• 作者: Fujita, H;Koshida, K;Mizokami, A
• 通讯作者: Mizokami, A