Functional analysis of cholesterol sulfate in interaction between embryo and endometrium.

我们研究了胚胎着床到子宫内膜的时空调控，发现在着床期子宫内膜中硫酸胆固醇（cholesterol sulfate，CS）含量短暂增加，仅在着床周围出现，CS抑制滋养细胞向子宫内膜的侵袭。本研究旨在探讨CS合成在胚胎发育过程中与子宫内膜相互作用的机制。1. CS抑制子宫内膜细胞培养液中MMP活性和纤溶酶活性。我们认为CS在子宫内膜异位症的发生过程中对体内蛋白酶活性的调节起着重要作用。2. CS促进了子宫内膜细胞中ROR靶基因RevErb的基因表达。此外，CS还能促进CS受体ROR基因的表达。3.在滋养层细胞系JEG中，CS刺激孕酮的产生。我们回顾了CS对与孕酮产生相关的酶的基因表达的作用，表明对它们没有影响。现在我们正在研究CS对胆固醇转运蛋白的亲和力。

We have studied on a tempo-spatial control of embryo implantation to uterine endometrium, demonstrating that the content of cholesterol sulfate(CS) in endometrium increases transiently during implantation period, the increase of CS is observed only in peri-implantation region, and CS inhibits the invasion of trophoblast to endometrium. In this study, we aimed to elucidate the function of CS synthesis in interaction between embryo and endometrium during implantation.1.It became clear that CS inhibit MMP activity as well as plasmin activity in the culture medium of endometrial cell culture system. We suggest that CS plays an important role in regulating a protease activity in vivo during implantation.2.CS promoted gene expression of RevErb regarded as a target gene of ROR in an endometrial cell. In addition, CS promoted gene expression of ROR which was the receptor of CS.3.In JEG which was cell line of trophoblast, CS stimulated a progesterone production. We reviewed action of CS on gene expression of enzymes associated with a progesterone production, suggesting no effect on them. Now we are examining the affinity of CS for cholesterol transportation protein.