Regulation and physiological function of sulfation of lipids in uterine endometrium during implantation period

着床期子宫内膜脂质硫酸化的调控及生理功能

• 批准号: 08671871
• 负责人: MOMOEDA Mikio
• 金额: $ 1.6万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671871/
• 关键词: endometrium; cholesterol sulfate; implantation; tropoblast; protease; 硫酸化脂質; スルファチド

As the basic research for the study of embryo implantation, we analyzed the changes in lipid components in the endometrium during the implantation period. Characteristic expression of cholesterol sulfate (CS) was observed in the rabbit endometrium during peri-implatation period. In present study, we found that cholesterol sulfotransferase was also activated during implantation period, suggesting that the enzyme upregulate the expression of CS. We succeeded to purify the cholesterol sulfotransferase, and are working on clonig the enzyme. As for the physiological function, we examined the effects of CS on trophoblast spreading in vitro and activity of proteases associated with the invasion of trophoblast. It was demonstrated that CS coated on the culture dish suppressed the trophoblast spreading and that CS inhibited the activity of plasmin among the various proteases. These results suggested that CS could regulate the invasion and growth of balstcyst at the implantation site.

作为胚胎着床研究的基础研究，我们分析了着床期间子宫内膜脂质成分的变化。孕周兔子宫内膜观察到硫酸胆固醇（CS）的特征性表达。在本研究中，我们发现胆固醇磺基转移酶在植入期间也被激活，表明该酶上调了CS的表达。我们成功地纯化了胆固醇磺基转移酶，并正在进行酶的克隆。在生理功能方面，我们检测了CS对滋养细胞体外扩散的影响以及与滋养细胞侵袭相关的蛋白酶活性。结果表明，培养皿上包被的CS抑制了滋养细胞的扩散，并抑制了各种蛋白酶中纤溶酶的活性。上述结果提示，CS可调节囊泡着床部位的侵袭和生长。

项目成果

Momoeda M,et al.: "Hormone dependent sulfation of lipid in uterine endometrium." In vitro Biology of Sex Steroid Hormone Action.112-119 (1996)

Momoeda M 等人：“子宫内膜中脂质的激素依赖性硫酸化”。

百枝幹雄・武谷雄二: "着床における子宮内膜硫酸化脂質の役割"産婦人科の世界. 51. (1999)

Mikio Momoeda 和 Yuji Takeya：“子宫内膜硫酸化脂质在植入中的作用”《妇产科世界》51。（1999 年）

Momoeda M,et al.: "Sulfation of lipids in uterine endometrium during implantation period." Biology of Pregnancy. 65-73 (1996)

Momoeda M 等人：“着床期间子宫内膜中脂质的硫酸化。”

百枝幹雄・武谷雄二: "子宮内膜制*機構in新女性医学大系12"中山書店. 401 (1998)

Mikio Momoeda 和 Yuji Taketani：“新女性医学系统中的子宫内膜异位症*机制 12”Nakayama Shoten 401 (1998)。

Momoeda, M. and Taketani, Y.: "Physiological function of sulfated lipids in uterin endometrium during inplantation period."Sanfujinka no Sekai. 51. (1999)

Momoeda, M. 和 Taketani, Y.：“着床期间子宫内膜硫酸化脂质的生理功能。”Sanfujinka no Sekai。