Regulation and physiological function of sulfation of lipids in uterine endometrium during implantation period

作为胚胎着床研究的基础研究，我们分析了着床期子宫内膜脂质成分的变化。观察了兔着床期子宫内膜中硫酸胆固醇(CS)的表达特点。在本研究中，我们发现胆固醇磺基转移酶在着床期也被激活，这表明该酶上调了CS的表达。我们成功地纯化了胆固醇磺酸转移酶，并正在对该酶进行克隆。在生理功能方面，我们检测了CS对滋养层细胞体外伸展的影响以及与滋养层细胞侵袭相关的蛋白水解酶活性。结果表明，涂布在培养皿上的壳聚糖能抑制滋养层细胞的铺展，并能抑制各种酶中的纤溶酶活性。这些结果表明，CS对种植部位的气囊侵袭和生长有调节作用。

As the basic research for the study of embryo implantation, we analyzed the changes in lipid components in the endometrium during the implantation period. Characteristic expression of cholesterol sulfate (CS) was observed in the rabbit endometrium during peri-implatation period. In present study, we found that cholesterol sulfotransferase was also activated during implantation period, suggesting that the enzyme upregulate the expression of CS. We succeeded to purify the cholesterol sulfotransferase, and are working on clonig the enzyme. As for the physiological function, we examined the effects of CS on trophoblast spreading in vitro and activity of proteases associated with the invasion of trophoblast. It was demonstrated that CS coated on the culture dish suppressed the trophoblast spreading and that CS inhibited the activity of plasmin among the various proteases. These results suggested that CS could regulate the invasion and growth of balstcyst at the implantation site.