The Stiffness Change of Conditioning Effect in Inner Hair Cells

内毛细胞调理效果的刚度变化

• 批准号: 17591774
• 负责人: YOSHIDA Naohiro
• 金额: $ 2.3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591774/
• 关键词: acoustic trauma; heat stress; hair cell

Overexposure to intense sound damages the inner ear cells can lead to permanent threshold shift (PTS). Conditioning effect can protect the ear from acoustic injury. The mechanism of this conditioning effect has been still unknown.In this research project, the mechanism of conditioning effect has been clarified by using 1) stiffness measurement by atomic force microscopy, 2) histological findings of f-actin and prestin by confocal microscope, 3) electrophysiological assay by DPOAE measurement. CBA strain mice were used in this project. The stiffness of outer hair cells has been elevated at 3, 6 hours after heat stress (41.5 degree, 15min). Furthermore, f-actin also has been elevated for 12 hours after heat stress. On the other hand, prestin which is the specific motor protein in outer hair cells, has not been elevated after heat stress. The enhancement of DPOAEs input-output function were observed in the same time, 3 to 6 hours after heat stress. PTS was protected dramatically at 6hours heat stress-trauma interval. These results would show the mechanism of conditioning effect by changing the stiffness with f-actin in inner hair cells.

过度暴露于强烈的声音会损害内耳细胞会导致永久阈值移位（PTS）。调节作用可以保护耳朵免受声学损伤。这种调节作用的机制仍然未知。在这项研究项目中，通过使用1）使用原子力显微镜测量刚度测量，2）共焦显微镜对F-肌动蛋白和PRESTIN的组织学发现，3）通过DPOAE测量进行了电生理学测定。该项目使用了CBA菌株小鼠。热应激后3、6小时（41.5度，15分钟）的3个小时，外毛细胞的刚度已升高。此外，热应激后，F-肌动蛋白也已升高12小时。另一方面，Prestin是外毛细胞中特定的运动蛋白，在热应激后尚未升高。在热应激后3至6小时，观察到DPOAES输入输出功能的增强。在6小时的热应力 - 间隔内，PTS受到巨大保护。这些结果将通过改变内毛细胞中F-肌动蛋白的刚度来表明调节作用的机理。

项目成果

Recent findings in acoustic trauma

声损伤的最新发现

• 发表时间: 2007
• 期刊: Jibiinnkoukarinsyou 100(5)
• 作者: Yoshida;N. et al.
• 通讯作者: N. et al.

音響外傷に関する最近の知見

关于声损伤的最新发现

• 发表时间: 2007
• 期刊: 耳鼻咽喉科臨床 100(5)
• 作者: Yoshizaki;T.;Wakisaka;N.;Pagano;JS;吉田 尚弘 他
• 通讯作者: 吉田 尚弘 他

Local mechanical properties of mouse outer hair cells. Atomic force microscopic study.

小鼠外毛细胞的局部机械特性。

• 发表时间: 2006
• 期刊: Auris Nasus Larynx 33(2)(印刷中)
• 作者: Murakoshi;M. et al.
• 通讯作者: M. et al.

Local mechanical properties of mouse outer hair cells; atomic force microscopic study.

小鼠外毛细胞的局部机械特性；

• 发表时间: 2006
• 期刊: Auris Nasus Larynx 33(2)
• 作者: Murakoshi;M. et al.;Murakoshi et al.
• 通讯作者: Murakoshi et al.

音響外傷に関する最新の知見

声损伤最新知识

• 发表时间: 2007
• 期刊: 耳鼻咽喉科臨床 100(5)(印刷中)
• 作者: 吉田 尚弘;小林俊光
• 通讯作者: 小林俊光