Analysis of keloid in an organotypic culture wound healing model

1.从耳垂瘢痕疙瘩中分离出成纤维细胞，制作成真皮模型(收缩胶原凝胶)，并制作成测量收缩过程及其稠度(振动阻抗)的模型系统。将瘢痕疙瘩真皮模型与正常皮肤来源成纤维细胞模型进行比较。收缩过程较快，真皮模型收缩较重。加入瘢痕疙瘩药物曲安奈德的培养液可抑制瘢痕疙瘩的收缩过程和稠度(对于正常成纤维细胞，稠度为常数)。早期加入曲安奈德制成的瘢痕疙瘩真皮模型的一致性与正常真皮模型无显著差异。此外，在培养第5天后在培养基中研磨曲安奈德，第10天对培养的一致性没有显著影响。我们证明了有一项工作可以抑制胶原蛋白凝胶的收缩并抑制…结论：1.进一步提高曲安奈德收缩胶原凝胶的稠度。用瘢痕疙瘩真皮替代物(模型)制作瘢痕疙瘩创面愈合模型。瘢痕疙瘩创面愈合模型表现为表皮层重建，与正常创面愈合模型相似。免疫组织化学染色未显示出与一般分化标记物的表现有显著差异。此外，我们制作了皮肤模型，我们使用无结构真皮(脱细胞真皮基质)来去除成纤维细胞。在无结构真皮创面愈合模型中，表皮成层不足。创面愈合模型中表皮的重建也不充分，尤其是基底层和上基底层的形成较差，向角化层的分化是病理性的。对于表皮化，成纤维细胞的存在比成纤维细胞的属性更多。在皮肤模型中，我们从神经纤维瘤的斑点中获得的成纤维细胞比正常皮肤来源的成纤维细胞更能增加皮肤模型中的色素水平。皮肤模型对于分析系统是有用的，以审查表皮和真皮对细胞间相互作用的影响。较少

1. We obtained fibroblasts from ear lobe keloid and made a corium model (contracted collagen gel) and made the model system to measure the course of the contraction and its consistency (impedance by vibration). A keloid corium model was compared with a model by normal skin origin fibroblasts. The contraction process occurred rapidly and corium model was contracted harder. The contraction process and consistency were inhibited by addition to culture media of the triamcinolone which was drug of keloid (with normal fibroblasts, as for the consistency, constant). As for the consistency of the keloid corium model that we made by adding triamcinolone in culture media in the early phase, there was not the significant difference for a normal corium model. In addition, it did not show a significant difference to consistency of culture the tenth day by grinding triamcinolone in culture media after culture the fifth day. We proved that there was a work to inhibit that collagen gel shrink and to d … More ecrease the consistency of contracted collagen gel in triamcinolone.2. Using a keloid dermal substitute (model), we made a keloid wound healing model. As for the keloid wound healing model, it revealed reconstitution of the epidermis layer which was similar to a normal wound healing model. Immunostaining did not show the remarked difference for manifestation of a general differentiation marker. In addition, we made the skin model that we used structurelessness corium (Acellular Dermal Matrix) which removed fibroblasts from. In a wound healing model by structurelessness corium, the layer formation of epidermis was insufficient. And the reconstitution of epidermis in a wound healing model was insufficient likewise, especially the formation of basal and suprabasal layers were bad, and differentiation to a cornified layer was pathognomonic. For epidermization, it was suggested that fibroblastic presence was massive than fibroblastic attribute.3. Pigment level increased in a skin model by the fibroblasts which we obtained from cafe au lait spots of neurofibroma, more than in a skin model by fibroblasts of a normal skin origin. Skin model was useful for a analysis system to review cell-cell interactional influence of epidermis and corium. Less