Experimental study of chronic skin ulcer re-epithelization by organotypic culture

本文利用三维皮肤培养模型和创面愈合模型，对由正常皮肤成纤维细胞和慢性溃疡成纤维细胞组成的皮肤等效模型在上皮形成和再上皮化过程中的差异进行了综述。慢性溃疡成纤维细胞增殖能力差，上皮形成和再上皮化过程均不充分。我们使用的替代系统是使用多代成纤维细胞或含有氢化可的松的培养液。在皮肤等效模型中，收缩过程和硬度被抑制。在皮肤培养模型和伤口愈合模型中，上皮细胞的形成和再上皮化过程受到抑制。

Using a three-dimensional skin culture model and a wound healing model, we tried to review difference in epithelium formation and re-epithelizaion process on skin equivalent model consist of fibroblasts which were obtained from normal skin and chronic ulcer. Fibroblasts obtained from chronic ulcer showed poor proliferative capacity, and both epithelium formation and re-epithelizaion process were insufficient. We used a substitute system that we use multiple passaged fibroblasts or hydrocortisone containing culture medium. In a skin equivalent model, the contraction process and the hardness were inhibited. And in a skin culture model and wound healing model, epithelium formation and re-epithelizaion process were restrained.