Basic research for fetal cardiac surgery

胎儿心脏外科基础研究

• 批准号: 17591865
• 负责人: MASUDA Munetaka
• 金额: $ 2.18万
• 依托单位: YOKOHAMA CITY UNIVERSITY (2006)Kyushu University (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591865/
• 关键词: fetal cardiac surgery; fetal cardiac bypass; umbilical artery; apoptosis; 胎児; 先天性心臓奇形; 体外循環; 心臓手術; 胎盤機能; 胎児循環

Background : To establish the safe fetal cardiac bypass is mandatory to perform the fetal cardiac surgical interventions. We investigated apoptosis in umbilical vessels related to fetal cardiac bypass. Methods : Thirteen pregnant ewes were used in the present study. Fetal lambs were divided into the cardiac bypass (CB) group (n=7) and the control group (n=6). In the CB group, cardiac bypass was maintained for 30 minutes using a centrifugal pump. After bypass, the fetuses were observed for 60 minutes and then the umbilical artery was harvested. In the control group, sham operation was performed and the umbilical artery was harvested. Umbilical artery tissue samples were used for TUNEL study and western blotting for Cytochrome c,Bax,Bcl-2,Bcl-xL, and Caspase 3. Results : The TUNEL-positive nuclei in umbilical artery were similar in both groups (CB group 0.57±0.29,control group 0.33±0.21 positive nuclei per high-power field, p=0.37). There were no significant differences in total caspase 3 levels between the 2 groups (CB group 1.40±0.43,control group 0.99±0.09,p=0.39). Cleaved caspase 3 were not detected in both groups. There were no significant differences in cytosolic cytochrome c levels between the 2 groups (CB group 1.99±0.75, control group 2.80±1.41,p = 0.60). There were no significant differences in Bax/Bc1-2 ratio between the 2 groups (CB group 1.66±0.66,control group 1.49±0.32,p = 0.83). There were no significant differences in Bcl-xL levels between the 2 groups (CB group 0.59±0.07,control group 0.58±0.10,p=0.93). Conclusions : We have demonstrated that fetal CB have not triggered additional apoptotic signals in the umbilical artery, suggesting that umbilical vessels may be patent after fetal cardiac surgery

背景：建立安全的胎儿心脏搭桥是进行胎儿心脏外科手术的强制性要求。我们研究了与胎儿心脏搭桥相关的脐血管细胞凋亡。方法：本研究使用 13 只怀孕母羊。胎羔分为心脏搭桥（CB）组（n=7）和对照组（n=6）。在CB组中，使用离心泵将心脏搭桥维持30分钟。搭桥后，观察胎儿60分钟，然后采集脐动脉。对照组进行假手术并摘取脐动脉。脐动脉组织样本用于 TUNEL 研究和蛋白质印迹法检测细胞色素 c、Bax、Bcl-2、Bcl-xL 和 Caspase 3。 结果：两组脐动脉中 TUNEL 阳性细胞核相似（CB 组每个高倍视野有 0.57±0.29 个阳性细胞核，对照组每个高倍视野有 0.33±0.21 个阳性细胞核，p=0.37）。两组间总caspase 3水平无显着差异（CB组1.40±0.43，对照组0.99±0.09，p=0.39）。两组中均未检测到裂解的 caspase 3。 2组间胞质细胞色素c水平无显着差异（CB组1.99±0.75，对照组2.80±1.41，p = 0.60）。 2组间Bax/Bc1-2比值无显着性差异（CB组1.66±0.66，对照组1.49±0.32，p=0.83）。 2组间Bcl-xL水平无显着差异（CB组0.59±0.07，对照组0.58±0.10，p=0.93）。结论：我们已经证明胎儿CB没有在脐动脉中触发额外的细胞凋亡信号，这表明胎儿心脏手术后脐血管可能是开放的