权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Interleukin-8 production from human umbilical vein endothelial cells during brief hyperglycemia : The effect of tumor necrotic factor alpha

短暂高血糖期间人脐静脉内皮细胞产生白细胞介素 8：肿瘤坏死因子 α 的作用

基本信息

批准号：
17591897
负责人：
KINOSHITA Kosaku
金额：
$ 2.11万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Background.This study evaluated the changes in chemokine interleukin (IL)-8 production from endothelial cells under various hyperglycemic conditions and investigated whether the hyperglycemia associated with the acute inflammatory response could enhance the IL-8 production from the endothelial cells.Materials and Methods.Human umbilical endothelial cells (HUVECs) were seeded at a concentration of 1×105 cells/well and cultured. The culture medium was replaced with Medium 199 containing various concentrations of glucose (final glucose concentration of culture medium was 100, 200, 300, 400, 500 mg/dL ; n= 7 each) with or without 100 ng of TNF-alpha. After 12 or 24 h at 37℃, the supernatants were collected from the cultures and stored at-80℃ until cytokine assay. IL-8 levels of the samples from the supernatants were quantified using a commercially available ELISA kit.Results.The IL-8 production by the HUVECs was significantly higher in the high glucose culture than in the control culture (glucose concentration of 100 mg/dL) (P<0.05). Moreover, the hyperglycemia associated with elevated TNF-alpha was found to enhance the level of IL-8 production by the HUVECs cultured at all glucose concentrations and over both time courses, compared to the control (P<0.05).Conclusions.In this study we observed a significant augmentation of IL-8 production by endothelial cells during short-term hyperglycemia, and a similar but significant stronger augmentation was obtained through TNF treatment. These findings suggest that the hyperglycemia associated with acute inflammatory response after trauma may run into the patients at high risk for secondary tissue damage.

本研究评价了在不同高血糖条件下内皮细胞产生趋化因子白细胞介素（IL）-8的变化，并研究了与急性炎症反应相关的高血糖是否能增强内皮细胞产生IL-8。用含有各种浓度的葡萄糖（培养基的最终葡萄糖浓度为100、200、300、400、500 mg/dL ;各n= 7）的培养基199替换培养基，其中含有或不含有100 ng TNF-α。在37℃下储存12或24 h后，从培养物中收集上清液，并在-80 ℃下储存，直至进行细胞因子测定。结果：高糖培养的HUVECs产生IL-8的能力显著高于对照培养（葡萄糖浓度为100 mg/dL）（P<0.05）。此外，与对照组相比，发现与升高的TNF-α相关的高血糖症增强了在所有葡萄糖浓度下培养的HUVEC在两个时间过程中产生IL-8的水平。（P<0.05）.结论：在本研究中，我们观察到在短期高血糖期间内皮细胞产生IL-8的显著增加，通过TNF治疗获得类似但显著更强的增强。提示创伤后急性炎症反应引起的高血糖可能是继发性组织损伤的高危因素。