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Expression patterns of tight junction associated proteins, occludin and claudins in ameloblasts during amelogenesis of mouse incisor.

小鼠门牙成釉细胞中紧密连接相关蛋白、occludin 和claudins 的表达模式。

基本信息

批准号：
17591909
负责人：
YAMAMOTO Toshio
金额：
$ 2.24万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Tight junctions (TJs) function primarily as a barrier against paracellular transports between epithelial cells and are composed of TJ associated proteins, occludin (OLD) and claudins (CLDs). CLDs form a family with 24 members and show tissue or cell specific expression. Ameloblasts originated from oral epithelium form enamel, and enamel proteins and minerals are transported across the ameloblastic layer during amelogenesis. We immunohistochemically examined the distribution patterns of TJs and expression patterns of OLD and CLDs (CLD-1 to CLD-10) in the ameloblasts. Secretory ameloblasts showed OLD, CLD-1, -8 and -9 at the distal end of the cell. In mature ameloblasts, OLD, CLD-1, -6, -7, -8, -9 and -10 were shown mainly at both distal and proximal ends of the cell, regardless of ruffle-ended or smooth-ended ameloblasts. The mature ameloblasts that only proximal ends were positively stained by these OLD and CLDs were also found. These results indicate that ameloblasts drastically change the expression patterns of CLDs and the distribution patterns of TJs between secretory and mature ameloblasts, and suggest that these differences reflect the cell functions of these cell types, specifically the transports of proteins and ions for enamel formation.

紧密连接（TJ）主要作为上皮细胞之间的细胞旁转运的屏障起作用，并且由TJ相关蛋白、闭合蛋白（OLD）和密蛋白（CLD）组成。CLD形成具有24个成员的家族，并且显示组织或细胞特异性表达。成釉细胞起源于口腔上皮细胞，形成釉质，釉质蛋白和矿物质在成釉细胞层中转运。我们用免疫组织化学方法检测了成釉细胞中TJ的分布模式以及OLD和CLD（CLD-1至CLD-10）的表达模式。分泌型成釉细胞在细胞远端显示OLD、CLD-1、-8和-9。在成熟的成釉细胞中，OLD、CLD-1、CLD-6、CLD-7、CLD-8、CLD-9和CLD-10主要分布于细胞的近端和远端，而无论是皱褶端还是光滑端的成釉细胞。这些结果表明，成釉细胞显著改变了分泌型成釉细胞和成熟型成釉细胞的CLDs表达模式和TJs分布模式，并提示这些差异反映了这些细胞类型的细胞功能，特别是蛋白质和离子的转运以形成釉质。