The study for functional correlation between palatability and gastric motility induced by appetite-enhancing peptides.

食欲增强肽诱导的适口性和胃动力之间功能相关性的研究。

• 批准号: 17591936
• 负责人: KOBASHI Motoi
• 金额: $ 2.18万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591936/
• 关键词: ghrelin; neuropeptide Y; melanin-concentrating hormone; stomach; gastric relaxation; palatability; medulla; vagus; Y1受容体; (D-Lys3)GHRP-6

The functional correlation between palatability and gastric motility induced by appetite-enhancing peptides were investigated in this study. The effects of neuropeptide Y (NPY) on motility of the proximal stomach were examined in anaesthetized rats. The administration of NPY into the 4th ventricle induced relaxation of the proximal stomach in a dose-dependent manner. The administrations of Y1 receptor (Y1R) agonist induced relaxation potently rather than those of NPY. The microinjections of Y1R agonist into the caudal part of the dorsal vagal complex (c-DVC) induced relaxation of the proximal stomach. The administration of NPY into the 4th ventricle did not induce relaxation after bilateral injection of Y1R antagonist into the c-DVC. These results revealed that NPY induced the relaxation in the proximal stomach via Y1R situated in the DVC. The effect of ghrelin on motility of the proximal stomach was also examined. The administration of ghrelin into the fourth ventricle induced relaxat … More ion of the proximal stomach in a dose-dependent manner. Simultaneous administration of ghrelin and GHS-R antagonist did not induce significant change in IGP. Microinjections of ghrelin into the c-DVC induced relaxation of the proximal stomach. These results indicate that ghrelin induces the relaxation of the proximal stomach via the GHS-R situated in the DVC as well as NPY. These results strongly suggest that the appetite-enhancing peptide facilitate reservoir function of the stomach to accommodate body to overconsumption elicited by the appetite-enhancing neuropeptides. However, the administration of the other appetite-enhancing peptides, melanin-concentrating hormone (MCH), did not induce gastric response. Intracerebroventricular (ICV) administrations of orexin, MCH and NPY increased the intake of saccharin. Drinking of saccharin in turn elevated the mRNA levels of orexin and NPY, but not MCH. Pre-treatments of naloxone, an opioid antagonist, blocked the orexigenic effects of orexin and NPY. These results suggest that the overconsumption promoted by sweet and palatable tastes is attributed to the activation of orexigenic neuropeptides, such as orexin and NPY, and a downstream opioid system together with enhanced digestive functions. Less

本研究调查了食欲增强肽诱导的适口性和胃动力之间的功能相关性。在麻醉大鼠中检查神经肽 Y (NPY) 对近端胃运动的影响。将 NPY 注入第四脑室以剂量依赖性方式诱导近端胃松弛。 Y1 受体 (Y1R) 激动剂的给药比 NPY 更能有效地诱导松弛。将 Y1R 激动剂显微注射到背侧迷走神经复合体 (c-DVC) 的尾部，诱导近端胃松弛。将 Y1R 拮抗剂双侧注射到 c-DVC 后，将 NPY 注射到第四心室不会引起舒张。这些结果表明，NPY 通过位于 DVC 中的 Y1R 诱导近端胃的松弛。还检查了生长素释放肽对近端胃运动的影响。将生长素释放肽注入第四脑室会以剂量依赖性方式诱导近端胃的松弛。同时给予ghrelin和GHS-R拮抗剂并没有引起IGP的显着变化。将胃饥饿素显微注射到 c-DVC 中可引起近端胃的松弛。这些结果表明，生长素释放肽通过位于 DVC 和 NPY 中的 GHS-R 诱导近端胃的松弛。这些结果强烈表明，食欲增强肽促进胃的储存功能，以适应身体对食欲增强神经肽引起的过度消耗。然而，服用其他增强食欲的肽——黑色素浓缩激素（MCH），并不会引起胃部反应。脑室内（ICV）施用食欲素、MCH 和 NPY 会增加糖精的摄入量。饮用糖精反过来会升高食欲素和 NPY 的 mRNA 水平，但不会升高 MCH。纳洛酮（一种阿片类拮抗剂）的预处理可阻断食欲素和 NPY 的促食欲作用。这些结果表明，甜味和可口味道促进的过度消费归因于促食欲神经肽（例如食欲素和 NPY）的激活以及下游阿片类系统以及增强的消化功能。较少的

项目成果

Central neuropeptide Y induces proximal stomach relaxation via Y1 receptors in the dorsal vagal complex of the rat.

中枢神经肽 Y 通过大鼠背侧迷走神经复合体中的 Y1 受体诱导近端胃松弛。

• 发表时间: 2006
• 期刊: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 290・2
• 作者: Hitoshi Yamamoto;Jinglei Cai;Rumi Yokota;Kunihiro Suzuki;Hideki Chisaka;Han-Sung Jung;Yukishige Kozawa;Kobashi M.;Kobashi M
• 通讯作者: Kobashi M

Possible role of orexigenic neuropeptides in the reservoir function of the stomach

促食欲神经肽在胃储存功能中的可能作用

• 发表时间: 2007
• 期刊: Neuropeptide Research Trends
• 作者: Hitoshi Yamamoto;Jinglei Cai;Rumi Yokota;Kunihiro Suzuki;Hideki Chisaka;Han-Sung Jung;Yukishige Kozawa;Kobashi M.
• 通讯作者: Kobashi M.

グレリン中枢投与による胃近位部食物受け入れ機能の促進作用

胃饥饿素的中枢给药促进近端胃的食物接受功能

• 发表时间: 2006
• 期刊: 日本味と匂学会誌 13・3
• 作者: Hitoshi Yamamoto;Jinglei Cai;Rumi Yokota;Kunihiro Suzuki;Hideki Chisaka;Han-Sung Jung;Yukishige Kozawa;Kobashi M.;Kobashi M;Furudono Y;小橋基
• 通讯作者: 小橋基

Central ghrelin induces gastric relaxation of the proximal stomach in the rats.

中央胃饥饿素诱导大鼠近端胃的胃松弛。

• 发表时间: 2006
• 期刊: Japanese Journal of Taste and Smell Research 13-3
• 作者: Kobashi M.;Xuan S.-Y.;Yanagihara M.;Mitoh Y.;Matsuo R.
• 通讯作者: Matsuo R.

Involvement of apecific orexigenic neuropeptides in sweetener-induced overconsumption in rats.

特定的促食欲神经肽参与甜味剂诱导的大鼠过度消费。

• 发表时间: 2006
• 期刊: Bahavioural Brain Research 175・2
• 作者: Kobashi M.;Xuan S.-Y.;Yanagihara M.;Mitoh Y.;Matsuo R.;Kobashi Motoi;Furudono Yuichi
• 通讯作者: Furudono Yuichi