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Study for autonomic response induced by emetic stimuli and their central neural mechanisms in rats

催吐刺激引起的大鼠自主反应及其中枢神经机制的研究

基本信息

批准号：
13671937
负责人：
KOBASHI Motoi
金额：
$ 1.92万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671937/
关键词：
apomorphine gastric motility vagal nerve submandibular salivary secretion neurokinine-1 rats vomiting nausea 顎下腺唾液分泌

项目摘要

The effects of apomorphine administration on the autonomic responses were investigated in rats. Distinctive gastric motor responses were observed after the intravenous administration of apomorphine (0.1 mg/kg body wt). Gastric motor responses in the distal stomach induced by apomorphine administration were classified into two types. One type involved inhibition of phasic contractions which appeared just after the administration of apomorphine. The other involved an increase in the frequency of small phasic contractions accompanied by increased gastric tone appearing with a relatively longer delay. No relaxation was observed in either the proximal or distal stomach. These gastric motor responses showed a dose-response effect to the amount of apomorphine administered (0.002 - 0.1mg/kg body wt). In addition, submandibular salivary secretion was observed in response to the intravenous administration of apomorphine at a dose of 3 or 10 mg/kg body wt. Pre-treatment with domperidone (1 or 2 m … More g/kg body wt) or the ablation of the area postrema (AP) abolished the gastric motor response and salivary secretion induced by the administration of apomorphine. In conclusion, rats showed definitive autonomic phenomena in response to the administration of apomorphine. Dopamine 2-like receptors situated in the AP mediate apomorphine-induced autonomic phenomena in rats. Pretreatment of intravenous NK-1 antagonist diminished the increase in intragastric pressure, dose-dependently. Microinjection of NK-1 antagonist into the vicinity of the nucleus ambiguus (NA) did not influence on the inhibitory response of the phasic contractions but diminished the increase in intragastric pressure. Microinjection of neurotoxic agents, ibotenic acid dissolved in pontamine sky blue solution, into the same area also diminished the increase in intragastric pressure. In conclusion, increase in intragastric pressure, which appeared with relatively longer delay after the apomorphine administration, may be mediated by NK1 receptors in the vicinity of the NA. Less

研究了阿波啡对大鼠自主神经反应的影响。静脉注射阿波啡（0.1 mg/kg体重）后，观察到明显的胃运动反应。阿波啡引起的远端胃运动反应可分为两种类型。一种类型涉及到在服用阿波啡后出现的相性收缩的抑制。另一种是小相位收缩的频率增加，伴随着胃张力的增加，出现的时间相对较长。胃近端和远端均未见松弛。这些胃运动反应显示出阿波啡给药量的剂量反应效应（0.002 - 0.1mg/kg体重）。此外，在静脉给药阿波啡3或10 mg/kg体重量后，观察到下颌下唾液分泌。预先用多潘立酮（1或2 m或更多g/kg体重量）或术后区域消融（AP）可消除阿波啡引起的胃运动反应和唾液分泌。综上所述，大鼠在阿波啡的作用下表现出明确的自主神经现象。位于AP中的多巴胺2样受体介导阿吗啡诱导的大鼠自主神经现象。静脉注射NK-1拮抗剂预处理降低了胃内压的升高，且呈剂量依赖性。微注射NK-1拮抗剂于二歧核（NA）附近，不影响相性收缩的抑制反应，但能降低胃内压的升高。将溶解于波他明天蓝溶液中的伊博tenic酸微量注射到同一区域，也能减少胃内压的升高。综上所述，阿波啡给药后较长时间出现的胃内压升高可能是由NA附近的NK1受体介导的。少