Gene expression of bone marrow cells from rats on HAP blocks and β-TCP blocks

本研究以HAP和β-TCP为支架材料，制备标准组织培养物作为对照，通过对HAP和β-TCP周围成骨情况的研究，以及细胞在HAP和β-TCP支架材料上的培养和基因表达谱的检测，为HAP和β-TCP修复颌骨缺损提供实验依据。骨髓细胞从大鼠培养后Maniatopoulos，等。等接种到材料和对照物上。接种后1、3、5天，从HAP和B-TCP块或塑料表面分离总RNA。从总RNA合成c-DNA后，使用大鼠寡核苷酸微阵列试剂盒（Agilent Technologies）制备标记的c-RNA，将其片段化并加入杂交混合物中。进行洗涤和染色，并使用Agilent Technologies微阵列扫描仪获取图像。用Gene Spring软件（Agilent Technologies）分析结果，HAP和β-TCP表面的多种基因表达与对照组相比，呈现各自特征性的变化。2-其中上调表达的基因包括凋亡组、骨形成或吸收相关组、细胞周期调控组、转录组等，早期上调表达的基因有与羟基磷灰石结合或钙离子结合相关的基因，这些结果表明，HAP和β-TCP块周围成骨的研究是可能的。

In this study, investigation of osteogenesis around HAP and β-TCP blocks, cell culture on the scaffold using with HAP and B-TCP blocks were carried out and gene expression profiles of them were examined for the purpose of the reconstruction of jaw bone using with HAP and β-TCP blocks.HAP and β-TCP blocks were used for scaffolds and standard plastic tissue culture were prepared for control. Bone marrow cells from rats cultured following Maniatopoulos, et. al. were seeded onto the materials and the control. 1, 3 and 5days after seeding, total RNA was isolated from HAP and B-TCP blocks or plastic surfaces. After c-DNA was synthesized from total RNA, labeled c-RNA was prepared, fragmented and added to a hybridization mixture using Rat Oligo Microarray Kit (Agilent Technologies). Washing and staining was done and images were acquired using the Agilent Technologies Microarray scanner. Gene Spring software (Agilent Technologies) was used to analyze the results.The expression of a variety of genes in HAP and β-TCP surfaces, as compared with the control group showed respective change characteristically. 2-fold up regulated genes of them were included in apoptosis group, bone formation or resorption-related group, cell cycle regulated group, transcription group and etc. Up regulated genes of B-TCP in early stage had the genes related to hydroxyapatite binding or calcium ion binding.From these results, it was suggested that investigation of osteogenesis around HAP and β-TCP blocks would be possible.